Anti-B-Raf (N581S) Mouse Monoclonal Antibody
英文名称： Anti-B-Raf (N581S) Mouse Monoclonal Antibody
型号:null    抗体货号： 26109
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

B-Raf (N581S) [26109]
[100 µL]		  

Catalog Number:  26109
Gene Symbol:  BRAF, BRAF1, RAFB1
Description:   Anti-B-Raf (N581S) Mouse Monoclonal Antibody
Background:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen:   A synthetic peptide from the internal region of Braf (N581S), human origin.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:2000 
IHC: 1:100-200
Concentration:   0.5 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes B-Raf N581S, but not wild-type B-Raf proteins from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant B-raf proteins. Purified His-tagged B-Raf (N581S) protein (amino acids 513-693, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-B-Raf (N581S) monoclonal antibody (Cat. # 26109).
Immunofluorescence:  
Immunofluorescence of cells expressing B-Raf proteins with anti-B-Raf (N581S) antibody. HEK293T cells were transfected with pCDNA3-GFP-B-Raf (WT) plasmid (left column) or pCDNA3-GFP-B-Raf (N581S) plasmid (right column), then fixed and stained with anti-B-Raf (N581S) monoclonal antibody (Cat. #26109).
Publications:
1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
    Mod Pathol. 2013 Mar;26(3):414-20.
2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
    Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
    human pathology. February 2015Volume 46, Issue 2, Pages 169–175
4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
    human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
5.  BRAF V600E Mutation-Specific Antibody: A Review
    Seminars in Diagnostic Pathology