Anti-BRAF (G469E) Mouse Monoclonal Antibody
英文名称： Anti-BRAF (G469E) Mouse Monoclonal Antibody
型号:null    抗体货号： 26196
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

BRAF (G469E) [26196]
[100ul]		  

Catalog Number:  26196
Gene Symbol:  BRAF, BRAF1, RAFB1
Description:   Anti-BRAF (G469E) Mouse Monoclonal Antibody
Background:    BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen:   A synthetic peptide from the internal region of BRAF which includes the mutation of G469E, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes G469E mutant, but not wild-type BRAF of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant BRAF (G469E) and wildtype proteins. Purified His-tagged BRAF (G469E) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-BRAF (G469E) monoclonal antibody (Cat. #26196).
Immunofluorescence:  
Immunofluorescence of cells expressing BRAF proteins with anti-BRAF (G469E) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid (left column) or pCDNA3-GFP-BRAF (G469E) plasmid (right column), then fixed and stained with anti-BRAF (G469E) monoclonal antibody (Cat. #26196).
Publications:
1.  Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
    Mod Pathol. 2013 Mar;26(3):414-20.
2.  Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
    Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733
3.  Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
    human pathology. February 2015Volume 46, Issue 2, Pages 169–175
4.  Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
    human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570
5.  BRAF V600E Mutation-Specific Antibody: A Review
    Seminars in Diagnostic Pathology