BRAF (V600A) [26199]
[100UL] | |
| Catalog Number: 26199 | | Gene Symbol: BRAF, BRAF1, RAFB1 | | Description: Anti-BRAF (V600A) Mouse Monoclonal Antibody | | Background: BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers. | | Immunogen: A synthetic peptide from the internal region of BRAF which includes the mutation of V600A, human origin. | | Applications: ELISA, WB, IHC | Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100 | | Concentration: 1 mg/ml | | Host: Mouse | | Format: Liquid | | Clonality: Monoclonal | | Isotype: | | Purity: Purified from ascites | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: Recognizes V600A mutant, but not wild-type BRAF of vertebrates. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Western blot: |  | Western blot analysis of recombinant BRAF proteins. Purified His-tagged BRAF (V600A) protein (lane 1) , corresponding wildtype protein (lane 2) , His-tagged BRAF (V600R) protein (lane 3), His-tagged BRAF (V600M) protein (lane 4), His-tagged BRAF (V600K) protein (lane 5), His-tagged BRAF (V600G) protein (lane 6), His-tagged BRAF (V600D) protein (lane 7) and His-tagged BRAF (V600E) protein (lane 8) were blotted with anti-BRAF (V600A) monoclonal antibody (Cat. #26199). | | Immunofluorescence of cells expressing BRAF proteins with anti-BRAF (V600A) antibody. HEK293T cells were transfected with pCDNA3-GFP-BRAF (WT) plasmid, pCDNA3-GFP-BRAF (V600A) plasmid, pCDNA3-GFP-BRAF (V600D) plasmid, pCDNA3-GFP-BRAF (V600G) plasmid pCDNA3-GFP-BRAF (V600M) plasmid or pCDNA3-GFP-BRAF (V600E) plasmid, then fixed and stained with anti-BRAF (V600A) monoclonal antibody (Cat. #26199). | | | Publications: | 1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
Mod Pathol. 2013 Mar;26(3):414-20. | 2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 | 3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
human pathology. February 2015Volume 46, Issue 2, Pages 169–175 | 4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 | 5. BRAF V600E Mutation-Specific Antibody: A Review
Seminars in Diagnostic Pathology | |
