Anti-EGFR(G719S) Mouse Monoclonal Antibody
英文名称： Anti-EGFR(G719S) Mouse Monoclonal Antibody
型号:null    抗体货号： 26083
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

EGFR (G719S) [26083]
[100 µL]		  

Catalog Number:  26083
Gene Symbol:  EGFR
Description:   Anti-EGFR(G719S) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase for ligands of the EGF family. EGFR activates several signaling cascades to convert extracellular cues into appropriate cellular responses. Defects in EGFR are associated with cancers such as lung cancer.
Immunogen:   A synthetic peptide from the internal region of human EGFR which includes the mutation of G719S.
Applications:   ELISA, WB, IHC, IF
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:1000-1:2000 
IHC: 1:50-1:1000 
IF: 1:50-1:100
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR(G719S), but not wild type EGFR protein of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant EGFR(G719S) and wild-type EGFR proteins. Purified his-tagged EGFR (G719S) protein (aa 702-987) (Cat. #10214) and wild-type EGFR protein (aa 702-987) (Cat. #10210) were blotted with anti-EGFR(G719S) mouse antibody (Cat. #26083).
Immunofluorescence:  
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (G719S) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (G719S) plasmid (right column), then fixed and stained with anti- EGFR (G719S) monoclonal antibody (Cat. #26083).
Publications:
1.  Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
    Tumor Biology. October 2014