Anti-BRCA1 (1720-1863) Mouse Monoclonal Antibody
英文名称： Anti-BRCA1 (1720-1863) Mouse Monoclonal Antibody
型号:null    抗体货号： 26351-2
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

BRCA1 (1720-1863) [26351-2]
[100ul]		  

Catalog Number:  26351-2
Gene Symbol:  BRCA1, BRCC1, IRIS, PNCA4, PPP1R53, PSCP
Description:   Anti-BRCA1 (1720-1863) Mouse Monoclonal Antibody
Background:   BRCA1 is normally expressed in cells of breast and other tissues, where it helps repair damaged DNA or destroy cells if DNA cannot be repaired. It is involved in the repair of chromosomal damage with an important role in the error-free repair of DNA double-strand breaks. If BRCA1 itself is damaged by a BRCA1 mutation, damaged DNA is not repaired properly, and this increases the risk for breast cancer.
Immunogen:   Purified BRCA1 (1720-1863) protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes BRCA1(1720-1863) of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant BRCA1 (1-120) and BRCA1 (1720-1863) proteins. Purified His-tagged BRCA1 (1-120) protein (lane1) and corresponding His-tagged BRCA1 (1720-1831) (lane2) were blotted with anti-BRCA1 (1720-1863) monoclonal antibody (Cat. #26351-2).
Immunofluorescence:  
Immunofluorescence of cells expressing BRCA1(1720-1863)protein with anti-BRCA1 (1720-1863) antibody. HEK293T cells was transfected with pCDNA3-GFP-BRCA1 (1720-1863) plasmid, then fixed and stained with anti-BRCA1 (1720-1863) monoclonal antibody (Cat. #26351-2).