| Catalog Number: 26081 | | Gene Symbol: IDH1 | | Description: Anti-IDH1 (R132H) Mouse Monoclonal Antibody | | Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. | | Immunogen: Recombinant protein of human IDH1 which includes the mutation of R132H. | | Applications: ELISA, WB, IHC, IP | Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100 | | Concentration: 1 mg/ml | | Host: mouse | | Format: Liquid | | Clonality: Monoclonal | | Isotype: IgG | | Purity: Purified from ascites | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: Anti-IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Western blot: |  | Western blot analysis of recombinant IDH1(R132H) and wild-type proteins. Purified His-tagged IDH1 (R132H) protein (Cat.#10199) and wild-type protein (amino acid 1-240) (Cat.#10198) were blotted with anti-IDH1(R132H) mouse antibody (Cat.# 26081)(Left panel) or with anti-IDH1 mouse antibody (Cat. #26136 right panel). | | Immunofluorescence: |  | Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1(R132H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH1 (WT) plasmid (left column) or pCDNA3-GFP-IDH1 (R132H) plasmid (right column), then fixed and stained with anti-IDH1 (R132H) monoclonal antibody (Cat. #26081). | |
Publications:
1. Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
Nature 513, 110–114 (04 September 2014)
3. Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015