Anti-IDH1 (R132P) Mouse Monoclonal Antibody
英文名称：  Anti-IDH1 (R132P) Mouse Monoclonal Antibody
型号:null    抗体货号： 26406
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

IDH1 (R132P) [26406]
[100ul]		  

Catalog Number:  26406
Gene Symbol:  IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH
Description:   Anti-IDH1 (R132P) Mouse Monoclonal Antibody
Background:   Isocitrate dehydrogenases (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Immunogen:   A synthetic peptide from the internal region of IDH1 which includes the mutation of R132P, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:100
Concentration:    1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  recognizes R132P mutant, but not wild-type IDH2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant IDH1 (R132P) and wildtype proteins. Purified His-tagged IDH1 (R132P) (lane 1) and corresponding wild-type IDH1 protein (lane 2) were blotted with anti-IDH1 (R132P) monoclonal antibody (Cat. #26406).
Immunofluorescence:  
Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1 (R132P) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH1(R132P) plasmid, pCDNA3-GFP-IDH1 (WT) plasmid , pCDNA3-GFP-IDH1(R132S) plasmid, pCDNA3-GFP-IDH1(R132L) plasmid , pCDNA3-GFP-IDH1(R132G) plasmid or pCDNA3-GFP-IDH1(R132C) plasmid , then fixed and stained with anti-IDH1 (R132P) monoclonal antibody (Cat. #26406).
Publications:
1.  Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
    Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2.  Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
    Nature 513, 110–114 (04 September 2014)
3.  Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
    Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015