Anti-JAK2 (R683G) Mouse Monoclonal Antibody
英文名称： Anti-JAK2 (R683G) Mouse Monoclonal Antibody
型号:null    抗体货号： 26314
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

JAK2 (R683G) [26314]
[100ul]		  

Catalog Number:  26314
Gene Symbol:  JAK2, JTK10, THCYT3
Description:   Anti-JAK2 (R683G) Mouse Monoclonal Antibody
Background:    JAK2 is a non-receptor tyrosine kinase. It is a number of the Janus kinase family and has been implicated in ssignaling by members of the type II cytokine receptor family, the GM-CSF receptor family, the gp130 receptor family, and the single chain receptors. JAK2 orthologs have been identified in all mammals for which complete genome genome data are available. Mutations of JAK2 have been implicated in polycythemia vera, essential thrombocythemia, and myelofibrosis as well as other myeloproliferative disorders.
Immunogen:   A synthetic peptide from the internal region of JAK2 which includes the mutation of R683G, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   0.5 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes R683G mutant, but not wild-type JAK2 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant JAK2 (R683G) and wildtype proteins. Purified His-tagged JAK2 (R683G) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-JAK2 (R683G) monoclonal antibody (Cat. #26314).
Immunofluorescence:  
Immunofluorescence of cells expressing JAK2 proteins with anti-JAK2 (R683G) antibody. HEK293T cells were transfected with pCDNA3-GFP-JAK2 (R683G) plasmid, pCDNA3-GFP-JAK2 (WT) plasmid, pCDNA3-GFP-JAK2 (R683S) plasmid or pCDNA3-GFP-JAK2 (R683T) plasmid, then fixed and stained with anti-JAK2 (R683G) monoclonal antibody (Cat. #26314).