Anti-CTNNB1 (T41A) Mouse Monoclonal Antibody
英文名称： Anti-CTNNB1 (T41A) Mouse Monoclonal Antibody
型号:null    抗体货号： 26305
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

CTNNB1 (T41A) [26305]
[100ul]		  

Catalog Number:  26305
Gene Symbol:  Beta-catenin, CTNNB, Catenin beta-1
Description:   Anti-CTNNB1 (T41A) Mouse Monoclonal Antibody
Background:   CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers.
Immunogen:   A synthetic peptide from the internal region of CTNNB1 which includes the mutation of T41A, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:2000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes T41A mutant, but not wild-type CTNNB1 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant CTNNB1 (T41A) and wildtype proteins. Purified His-tagged CTNNB1 (T41A) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-CTNNB1 (T41A) monoclonal antibody (Cat. #26305).
Immunofluorescence:  
Immunofluorescence of cells expressing CTNNB1 proteins with anti-CTNNB1 (T41A) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1 (T41A) plasmid (right column), then fixed and stained with anti-CTNNB1 (T41A) monoclonal antibody (Cat. #26305).