Anti-IDH2 (R172W) Mouse Monoclonal Antibody
英文名称： Anti-IDH2 (R172W) Mouse Monoclonal Antibody
型号:null    抗体货号： 26164
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

IDH2 (R172W) [26164]
[100 µL]		  

Catalog Number:  26164
Gene Symbol:  IDH2 (R172W)
Description:   Anti-IDH2 (R172W) Mouse Monoclonal Antibody
Background:   Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172W IDH2 mutations do exist in diffusely infiltrative gliomas.
Immunogen:   A synthetic peptide from the internal region of IDH2 which includes the mutation of R172W, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  recognizes IDH1(R172W) of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant IDH2 (R172W) and wildtype proteins. Purified His-tagged IDH2 (R172W) (lane 2) and wildtype protein (lane 1) were blotted with anti-IDH2 (R172W) monoclonal antibody (Cat. #26164).
Immunofluorescence:  
Immunofluorescence of cells expressing IDH2 proteins with anti-IDH2 (R172W) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2 (R172W) plasmid (right column), then fixed and stained with anti-IDH2 (R172W) monoclonal antibody (Cat. #26164).
Publications:
1.  Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
    Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2.  Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
    Nature 513, 110–114 (04 September 2014)
3.  Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
    Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015