sarl活性单克隆抗体
英文名称: Anti-Active Sar1 Mouse Monoclonal Antibody
型号:null    抗体货号: 26916
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

  •  
    Active Sar1-GTP [26916] 
    [30 µL]
     
    Catalog Number:  26916
    Gene Symbol:  SAR1
    Description:   Anti-Active Sar1 Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER.
    Immunogen:   Recombinant full length protein of active Sar1a.
    Applications:   IP, IHC
    Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
    1:100 dilution for IHC
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-active Sar1 antibody recognizes active Sar1 of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
    Download Datasheet:  
    Immunoprecipitation/Western blot:  
    Sar1 activation assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No. 26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody (Cat. No. 21090).

翁�;">本品是由R-藻红蛋白标记的山羊抗大鼠IgGH+LF(ab)2片段,是将山羊抗血清经胃蛋白酶消化并使用抗原偶联的琼脂糖微珠亲和色谱纯化所得,借此可去除Fc片段和完整IgG分子。免疫电泳和/ELISA法检测显示本品特异性结合完整的大鼠IgG分子,也会与其他大鼠免疫球蛋白的轻链结合。本品预先与相近物种包括人、牛、马、兔的血清蛋白经过亲和吸附处理,ELISA/或固相免疫吸附检测证实本品与以上物种几乎无交叉反应。但可能会与其他物种免疫球蛋白发生交叉反应。本品不会与非免疫球蛋白类的血清蛋白发生交叉反应。R-藻红蛋白的Amax(最大激发波长)为490nm,545nm,566nmEmax(最大发射波长)为580nm

本品可做多标实验,应用于流式细胞术(FC)等实验。

产品应用

建议稀释浓度:1:50-1:200For most application

产品性质

藻胆(色素)蛋白浓度

PhycobiliproteinConcentration

100µl (0.5mg/ml)

缓冲液(Buffer

0.01M ***酸钠,0.25M 氯化钠, pH 7.6

稳定剂(Stabilizer

15mg/ml BSA(无IgG,蛋白酶)

荧光素(Fluorophore

Phycoerythrin Amax=490nm,545nm,566nm;Emax=580nm

防腐剂(Preservative

0.05% 叠氮化钠

运输与保存方法

冰袋运输。4分装保存,切忌冻存!有效期6个月。

注意事项

1)本品含叠氮化钠,对人体有害,请注意适当防护。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。