Anti-EGFR(G719A) Mouse Monoclonal Antibody
英文名称： Anti-EGFR(G719A) Mouse Monoclonal Antibody
型号:null    抗体货号： 26115
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

EGFR (G719A) [26115]
[100 µL]		  

Catalog Number:  26115
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR(G719A) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of G719A, human origin.
Tested Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:    1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR(G719A), but not wild type EGFR of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant EGFR (G719A) and wildtype proteins. Purified His-tagged EGFR (G719A) protein (aa 702-987) and wild-type protein (aa 702-987) were blotted with anti- EGFR (G719A) mouse antibody (Cat. #26115).
Immunofluorescence:  
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (G719A) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (G719A) plasmid (right column), then fixed and stained with anti- EGFR (G719A) monoclonal antibody (Cat. #26115).
Publications:
1.  Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
    Tumor Biology. October 2014