| Catalog Number: 21281 | | Gene Symbol: STK11, LKB1, PJS, hLKB1 | | Description: Anti-STK11 Rabbit Polyclonal Antibody | | Background: Serine/threonine kinase 11 (STK11) also known as liver kinase B1 (LKB1) or renal carcinoma antigen NY-REN-19 is a kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response, and functions as a tumour suppressor. Recent studies have uncovered a large number of somatic mutations of the STK11 gene that are present in lung, cervical, breast, intestinal, testicular, pancreatic and skin cancer. | | Immunogen: Purified STK11 protein, human origin. | | Applications: ELISA, WB, IHC | Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200 | | Concentration: 0.5mg/ml | | Host: Rabbit | | Format: Liquid | | Clonality: Polyclonal | | Isotype: | | Purity: Purified from serum | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: Recognizes STK11 of vertebrates. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Western blot: |  | Western blot analysis of recombinant STK11 protein. Purified His-tagged STK11 protein was blotted with anti-STK11 rabbit polyclonal antibody (Cat. #21281). |  | Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue with anti-STK11 polyclonal antibody (Cat. #21281). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody. | | |