Anti-CTCF (R339W) Mouse Monoclonal Antibody
英文名称： Anti-CTCF (R339W) Mouse Monoclonal Antibody
型号:null    抗体货号： 26326
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

CTCF (R339W) [26326]
[100ul]		  

Catalog Number:  26326
Gene Symbol:  CTCF; MRD21
Description:   Anti-CTCF (R339W) Mouse Monoclonal Antibody
Background:   Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is atranscription factor that in humans is encoded by the CTCF gene. CTCF is involved in many cellular processes, including transcriptional regulation, insulator activity, recombination and regulation of chromatin architecture.
Immunogen:   A synthetic peptide from the internal region of CTCF which includes the mutation of R339W, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes R339W mutant, but not wild-type CTCF of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant CTCF (R339W) and wild-type proteins. Purified His-tagged CTCF (R339W) protein (lane 1) and corresponding wild-type protein (lane 2) were blotted with anti-CTCF (R339W) monoclonal antibody (Cat. #26326).
Immunofluorescence:  
Immunofluorescence of cells expressing CTCF proteins with anti-CTCF (R339W) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTCF (WT) plasmid (left column) or pCDNA3-GFP-CTCF (R339W) plasmid (right column), then fixed and stained with anti-CTCF (R339W) monoclonal antibody (Cat. #26326).