| Catalog Number: 26064 | | Gene Symbol: RAC3 | | Description: Anti-Rac3 Mouse Monoclonal Antibody | | Background: Rac3 is a small GTPase of the Rho family that relays signals through direct interaction of GTP-bound Rac3 with downstream effector molecules. The Rac subfamily consists of Rac1, Rac2, and Rac3 proteins. While Rac1 is ubiquitously expressed, Rac2 and Rac3 are largely restricted to hematopoietic and neural tissues, respectively. Rac3 has biochemical properties that are closely related to Rac1. Both Rac1 and Rac3 have been implicated in the regulation of cell migration and invasion in human metastatic cancer. | | Immunogen: A synthetic peptide near the C-terminus of human Rac3. | | Applications: ELISA, WB, IHC, IP | Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100 | | Concentration: 1 mg/ml | | Host: mouse | | Format: Liquid | | Clonality: Monoclonal | | Isotype: IgG | | Purity: Purified from ascites | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: Specifically recognizes Rac3, but not Rac1 or Rac2 of vertebrates. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Western blot: |  | Western blot analysis of recombinant Rac3 proteins. Purified his-tagged Rac1 (Cat. #10101), Rac2 (Cat. #10184), and Rac3 (Cat. #10182) proteins were blotted with anti-Rac3 monoclonal antibody (Cat. #26064). | | Publications: | 1. Phosphorylation of α-tubulin by protein kinase C stimulates microtubule dynamics in human breast cells
Cytoskeleton Volume 71, Issue 4, pages 257–272, April 2014 | 2. Immunofluorescence and Confocal Microscopy of Neutrophils
Neutrophil Methods and Protocols Methods in Molecular Biology Volume 1124, 2014, pp 251-268 | 3. A link between the nuclear-localized srGAP3 and the SWI/SNF chromatin remodeler Brg1
Molecular and Cellular Neuroscience Volume 60, May 2014, Pages 10–25 | 4. SHP2 Phosphatase Promotes Mast Cell Chemotaxis toward Stem Cell Factor via Enhancing Activation of the Lyn/Vav/Rac Signaling Axis
The Journal of Immunology April 14, 2014 1301155 | 5. ROBO1, a tumor suppressor and critical molecular barrier for localized tumor cells to acquire invasive phenotype: Study in African-American and Caucasian prostate cancer models
International Journal of Cancer Volume 135, Issue 11, pages 2493–2506, 1 December 2014 | 6. Transcription factors NRF2 and nf-kb are coordinated effectors of the RHO family, GTP binding protein rac1 during inflammation
J Biol Chem. 2014 May 30;289(22):15244-58 | 7. Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan
Mol Cell Biol. 2014 Nov 15;34(22):4186-99 | 8. p53 Down Regulates PDGF-Induced Formation of Circular Dorsal Ruffles in Rat Aortic Smooth Muscle Cells
PLoS One. 2014 Sep 23;9(9):e108257 | 9. LPA-mediated migration of ovarian cancer cells involves translocalization of Gαi2 to invadopodia and association with Src and β-pix
Cancer Lett. 2015 Jan 28;356(2 Pt B):382-91 | 10. cAMP controls the restoration of endothelial barrier function after thrombin-induced hyperpermeability via Rac1 activation
Physiological ReportsPublished 24 October 2014Vol. 2no. e12175DOI: 10.14814/phy2.12175 | 11. Cisplatin at Sub-toxic Levels Mediates Integrin Switch in Lung Cancer Cells
Anticancer Research December 2014 vol. 34 no. 12 7111-7117 | 12. Semaphorin-3F suppresses the stemness of colorectal cancer cells by inactivating Rac1
Cancer letters. March 1, 2015Volume 358, Issue 1, Pages 76–84. | 13. Geometry sensing through POR1 regulates Rac1 activity controlling early osteoblast differentiation in response to nanofiber diameter
Integr Biol (Camb). 2014 Dec 24 | 14. Inhibition of Rac1 Activity in the Hippocampus Impairs the Forgetting of Contextual Fear Memory
Mol Neurobiol. 2015 Jan 24 | | |