Background: Estrogen receptor (ER) is a member of the nuclear hormone family of intracellular receptors. There are two different forms of the estrogen receptor, usually referred to as α and β, each encoded by a separate genes (ESR1 and ESR2, respectively). Upon activation by estrogen, the estrogen receptor is able to bind to DNA and regulate the activity of many different genes. Dysfunction of Estrogen receptors are implicated to cause diseases such as cancer (breast cancer), aging, and obesity.
Immunogen: A synthetic peptide from the internal region of ERα, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes ERα protein from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of His-ERα protein . His-tagged ER protein (amino acids 1-300, Cat. #10251) was blotted with the polyclonal anti-ER1 antibody (Cat. #21220).