Anti-EGFR (V769L) Mouse Monoclonal Antibody
英文名称： Anti-EGFR (V769L) Mouse Monoclonal Antibody
型号:null    抗体货号： 26121
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

EGFR (V769L) [26121]
[100 µL]		  

Catalog Number:  26121
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR (V769L) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of V769L, human origin.
Tseted Applications:   ELISA, WB, If, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:2000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR (V769L), but not wild type EGFR from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant EGFR (V769L) and wildtype proteins. Purified His-tagged EGFR (V769L) protein (amino acids 702-987, lane 2, Cat. #10239) and corresponding wild type protein (lane 1, Cat. #10210) were blotted with anti-EGFR (V769L) mouse monoclonal antibody (Cat. #26121). Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. #28004).
Immunofluorescence:  
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (V769L) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (V769L) plasmid (right column), then fixed and stained with anti- EGFR (V769L) monoclonal antibody (Cat. # 26121).
Publications:
1.  Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
    Tumor Biology. October 2014