Anti-FGFR1 (P252R) Mouse Monoclonal Antibody
英文名称： Anti-FGFR1 (P252R) Mouse Monoclonal Antibody
型号:null    抗体货号： 26249
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

FGFR1 (P252R) [26249]
[100ul]		  

Catalog Number:  26249
Gene Symbol:  FGFR1, BFGFR, CD331, CEK, FGFBR, FLG
Description:   Anti-FGFR1 (P252R) Mouse Monoclonal Antibody
Background:   The protein FGFR1 is highly conserved between members and throughout evolution. This particular family member of FGFR1 binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations of FGFR1 protein have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome.
Immunogen:   A synthetic peptide from the internal region of FGFR1 which includes the mutation of P252R, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000 
WB: 1:500-1:1000 
IHC: 1:50-1:100
Concentration:    1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes P252R mutant, but not wild-type FGFR1 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant FGFR1 (P252R) and wildtype proteins. Purified His-tagged FGFR1 (P252R) protein (22-376aa, 45KD, lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-FGFR1 (P252R) monoclonal antibody (Cat. #26249).
Immunofluorescence:  
Immunofluorescence of cells expressing FGFR1 proteins with anti-FGFR1 (P252R) antibody. HEK293T cells were transfected with pCDNA3-GFP-FGFR1 (WT) plasmid (left column) or pCDNA3-GFP-FGFR1 (P252R) plasmid (right column), then fixed and stained with anti-FGFR1 (P252R) monoclonal antibody (Cat. #26249).
Publications:
1.  Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
    Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2.  Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
    Nature 513, 110–114 (04 September 2014)
3.  Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
    Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015