Anti-PIK3CA(E545K) Rabbit Polyclonal Antibody
英文名称： Anti-PIK3CA(E545K) Rabbit Polyclonal Antibody
型号:null    抗体货号： 21145
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

PIK3CA (E545K) [21145]
[100 µL]		  

Catalog Number:  21145
Gene Symbol:  PIK3CA(E545K)
Description:   Anti-PIK3CA(E545K) Rabbit Polyclonal Antibody
Background:   Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The catalytic subunit is encoded by the PIK3CA gene. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. The PIK3CA gene is one of the most frequently mutated genes in human cancers.
Immunogen:   A synthetic peptide from the internal region of PIK3CA(E545K) of human origin.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:100-1:5000 
WB: 1:100-1:1000 
IHC: 1:10-1:100
Concentration:   0.2 mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-PIK3CA(E545K) antibody recognizes PIK3CA(E545K) of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of PIK3CA wild type and E545K proteins. Equal amounts of purified PIK3CA(E545K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with anti-PIK3CA(E545K) rabbit polyclonal antibody (Cat. # 21145).