"抗体，钙调蛋白激酶激酶α抗体CaMKKα
产品货号：YM-S17343-X
规 格：0.1ml/0.2ml
浓 度：1mg/1ml.
抗体来源：Rabbit / mouse
克隆类型：兔来源为polyclonal，鼠来源为monoclonal
产品类型：一抗 单抗
性 状：冻干粉或液体
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
抗体，钙调蛋白激酶激酶α抗体CaMKKαRules:
（1） the initial reaction to produce antibodies to antigens: when thefirst to enter the body, need to produce antibodies after certainincubation period can, and antibody production quantity are not many, inthe body to maintain the time is short.
（2） once again: when the same antigen antibody reaction to producesecond times into the body, at the beginning, as part of the originalantibody binding and once again into the antigen antibody, can make theoriginal amount reduced slightly. Subsequently, rapid increase antibodytiter, several times more than the initial reaction can be generated toa few times, retained in the body of a lot of time.
（3） recall response to produce antibodies: antibodies produced byantigen stimulation of the body, after a certain period of time candisappear gradually. If the re exposure to antigen, the antibody hasfaded fast rise. As the body with the same antigen again first, thenknown as the specific recall responses; and if the initial reactions ofdifferent, it is known as a nonspecific recall response. Increasednonspecific memories to elicit antibody responses are temporary, shorttime soon fall.
抗体产品WB实验中的对照系统：
Western blotting实验原理：免疫印迹(immunoblotting)又称蛋白质印迹(Western blotting)，它是根据抗原抗体的特异性结合检测复杂样品中的某种蛋白的方法。该法是在凝胶电泳和固相免疫测定技术基础上发展起来的一种新的免疫生 化技术。由于免疫印迹具有SDS-PAGE的高分辨力和固相免疫测定的高特异性和敏感性，现已成为蛋白分析的一种常规技术。免疫印迹常用于鉴定某种蛋白， 并能对蛋白进行定性和半定量分析。
WB对照系统：阳性对照：最好有标准品，或阳性血清、阳性上清。
阴性对照：测血时用相应小鼠未免疫血清，测培养上清，用无克隆培养上清。空白对照：不加一抗，用128%BSA代替。无关对照（替代对照）：用无关项目一抗，或无关项目的抗体。
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