Anti-Arf1 Rabbit Polyclonal Antibody
英文名称: Anti-Arf1 Rabbit Polyclonal Antibody
型号:null    抗体货号: 21283
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Arf1 [21283]
[100 µL]
 

Catalog Number:  21283
Gene Symbol:  ARF1
Description:   Anti-Arf1 Rabbit Polyclonal Antibody
Background:   ARF1 (ADP-ribosylation factor 1) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport.
Immunogen:   Purified Arf1 protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:200
Concentration:   0.2mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes Arf1, Arf2 and Arf3 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Arf1 protein. Purified His-tagged Arf1 protein was blotted with anti-Arf1 rabbit polyclonal antibody (Cat. #21283).
Immunohistochemistry:  
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti-Arf1 polyclonal antibody (Cat. #21283). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.