B-Raf (V600E) [26039]
[100 µL] | |
| Catalog Number: 26039 | | Gene Symbol: B-Raf | | Description: Anti-B-Raf(V600E) Mouse Monoclonal Antibody | | Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers | | Immunogen: A synthetic peptide from the internal region of BRaf(V600E), human origin. | | Applications: ELISA, WB, IHC, IP | Recommended Dilutions:
ELISA: 1:100-1:5000
WB: 1:100-1:2000
IHC: 1:100-1:1000 | | Concentration: 0.5 mg/ml | | Host: mouse | | Format: Liquid | | Clonality: Monoclonal | | Isotype: IgG2b | | Purity: Purified from ascites | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: Recognizes B-Raf(V600E), but not wild type B-Raf proteins from vertebrates. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Western blot: |  | Western blot analysis of recombinant B-raf proteins with anti-BRaf(V600E) antibody. Purified His - tagged B-Raf( V600E ) ?amino acids 513-693)(lane1) ? corresponding wild type protein(lane 2), His - tagged B-Raf( V600D ) (lane 3) , His - tagged B-Raf( V600A ) (lane 4) , His - tagged B-Raf( V600G ) (lane 5) , His - tagged B-Raf( V600K ) (lane 6), His - tagged B-Raf( V600R ) (lane 7), and His - tagged B-Raf( V600M ) (lane 8) were blotted with anti-B-Raf(V600E) monoclonal antibody (Cat # 26039) . | | Immunofluorescence of cell expressing BRaf protein with anti-BRaf(V600E) antibody . HEK293T cells were transfected with pCDNA3-GFP- B-Raf( V600E )plasmid,pCDNA3-GFP- B-Raf( WT )plasmid, pCDNA3-GFP- B-Raf( V600K )plasmid,pCDNA3-GFP- B-Raf( V600R )plasmid, pCDNA3-GFP- B-Raf( V600A )plasmid,pCDNA3-GFP- B-Raf( V600D ) plasmid, pCDNA3-GFP-B-Raf(V600M)plasmid, or pCDNA3-GFP-B-Raf(V600G)plasmid, then fixed and stained with anti-B-Raf(V600E) monoclonal antibody (Cat. # 26039). |  | Immunohistochemical analysis of paraffin-embedded human melanoma tissue with anti-B-Raf (V600E) monoclonal antibody (Cat. # 26039). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody. | | | Publications: | 1. Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma
Mod Pathol. 2013 Mar;26(3):414-20. | 2. Immunohistochemistry as a quick screening method for clinical detection of BRAF(V600E) mutation in melanoma patients
Tumor Biology. June 2014, Volume 35, Issue 6, pp 5727-5733 | 3. Prospective immunohistochemical analysis of BRAF V600E mutation in melanoma
human pathology. February 2015Volume 46, Issue 2, Pages 169–175 | 4. Comparison of 2 monoclonal antibodies for immunohistochemical detection of BRAF V600E mutation in malignant melanoma, pulmonary carcinoma, gastrointestinal carcinoma, thyroid carcinoma, and gliomas
human pathology. November 2013Volume 44, Issue 11, Pages 2563–2570 | 5. BRAF V600E Mutation-Specific Antibody: A Review
Seminars in Diagnostic Pathology | |
