琼脂糖片剂
英文名称： InstantAgaros
型号:null    产品货号： R9012TAB
价格:请致电：010-57128832,18610462672
品牌: 进口

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Figure 2: Coomassie-stained SDS-PAGE analysis of different proteins expressed using the RTS 500 and 9000 E. coli HY Kits.
a: RTS 9000 E. coli HY expression; b: RTS 500 E. coli HY expression
CAT: Chloramphenicol Acetyltransferase; β-Gal: β-Galactosidase; GFP: Green Fluorescent Protein;
RHO: Rhodanese; rPA: recombinant Plasminogen Activator; CEC: GFP-(CecoprinP1)2;
PGA: Penicillin G Amidase, β-UE; LUC: Firefly Luciferase; MBP-EPO: MBP-***

Description

One RTS 9000 reaction produces ten times more total protein yield than a RTS 500 reaction. This higher total yield is achieved by increasing the reaction volume by a factor of ten, while the productivity per ml lysate in the RTS 500 HY and RTS 9000 HY reactions is similar (Figure 2).
The RTS 9000 Reaction Device (Figure 1) is composed of a reaction compartment and a feeding compartment. The 10-ml reaction compartment, where the coupled transcription/translation reaction takes place, is separated from the 100-ml feeding compartment by a semipermeable membrane (cut-off 10 kD). The plasmid that contains the gene of interest serves as a template for the transcription. An optimized E. coli lysate contains tRNAs, ribosomes, enzymes, factors, and other necessary components required for highly efficient translation. The feeding compartment continuously supplies ions, energy substrates, nucleotides, and amino acids through the semipermeable membrane. Simultaneously, by-products that may inhibit the reaction are diluted through the same membrane into the feeding compartment. This continuous exchange of substrates and by-products across the two semipermeable membranes (CECF technology) allows protein synthesis to continue for up to 24 hours.

Read more about RTS E.coli System and Technology

*See instrument compatibility table and the Eppendorf ThermoMixer Quickreference

Former Roche customers please click here to see the product code cross reference table.