111<br>试剂级别： 11111Brand,null 产品货号： 111Item#, 111<br>英文名称： 111

111
英文名称： 111

型号:null 产品货号： 111

价格:请致电：010-57128832,18610462672

品牌: 111
试剂级别： 11111

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Bacterial Strain BL21(DE3)pLysS, Glycerol Stock, noncompetent

Bacterial Strain BL21(DE3)pLysS(a)(细菌 BL21 (DE3)pLysS)可以在T7启动子启动下和核糖体结合位点的帮助下实现高效蛋白表达。 BL21(DE3)pLysS 对λ-DE3 具有溶源性(1)，含有 T7抗菌素基因I, 编码 T7 RNA聚合酶的基因(2) 并受到 lac UV5启动子的控制。BL21(DE3)pLysS 还含有质粒 pLysS, 携带编码 T7溶菌酶素的基因。T7溶菌酶素可以降低T7启动子控制下的目的基因的背景表达水平，但是不影响IPTG诱导下蛋白的产量。

基因型：F-, ompT, hsd SB (rB-,mB-), dcm, gal, λ(DE3), pLysS, Cmr.

特点

• T7 启动子引导的表达: 含有能被IPTG-诱导的在T7 RNA聚合酶基因。

操作手册 Technical Bulletin TB095

储存条件: 储存于-70℃。

参考文献1. Studier, F.W. and Moffatt, B.A. (1986) J. Mol. Biol. 189, 113–30.2. Davanloo, P. et al. (1984) Proc. Natl. Acad. Sci. USA 81, 2035–9.(a) Usage restrictions apply to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and toany derivatives thereof. Please read the statement in the Acknowledgments/Disclaimerssection in the Technical and Legal References chapter describing these restrictions beforepurchasing any of these products.

产品名称	目录号	数量	规格	产地	价格(元)
Bacterial Strain BL21(DE3)pLysS, Glycerol Stock, noncompetent	P9811	1	500μl	promega	301