美国Brand,null 产品货号： SL100489-SiHaItem#, 预优化DNA转染试剂-SiHa<br>英文名称： GenJet™ In Vitro DNA Tranfection Reagent for SiHa Cells

Description

GenJet™ DNA In Vitro Tranfection Reagent for SiHa is pre-optimized for transfecting SiHa cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on SiHa cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)

~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours

EGFP
pEGFP-N3 (CMV promoter)
76%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet.pdf