Lonza Nucleofector 细胞核转染试剂盒 VVPL-1004
英文名称： 100 RCT Mouse/Rat Hepatocyte Kit
型号:null    产品货号： VVPL-1004
价格:请致电：010-57128832,18610462672
品牌: 德国进口

北京泽平代理的Lonza Nucleofector 细胞核转染仪 (原名Amaxa Nucleofector)，通过电穿孔技术，配合专用电转染试剂盒，能将DNA、RNA等物质导入细胞，并直接穿过核膜进入细胞核，实现高效转染。北京泽平是Lonza中国区一级代理，销售Lonza旗下全系列产品，如Lonza Nucleofector细胞核转染系统、CytoSMART活细胞成像系统、原代细胞和培养基、基础细胞培养基（DMEM、MEM、RPMI 1640）、内毒素检测试剂盒、支原体检测试剂盒及清除试剂盒、琼脂糖和闪胶等。自2009年起北京泽平成为Lonza全国代理，凭借优异的产品和服务，受到广大客户的认可！欢迎来电咨询北京泽平官方客服400-686-0100或13910005869或13521452266

C-1/MAC-3 and CD68 by flow cytometry or immunohistochemical staining.

Size & content:
- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 25 pmoles siRNA or miRNA mimics in 24-well plate
- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution

Refer to the following optimal transfection conditions for maximal silencing on primary macrophages. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency 

Transfection Results:
Reporter Gene
Silencing Efficiency (% )


DMEM with 4.5 g/L glucose, 10% FBS
~90%
50 nM
1xGenMute™ Transfection Buffer
~15 minutes at RT
48 hours


Renilla Luciferase
85%


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol.pdf