Lonza Nucleofector 细胞核转染试剂盒 VSOP-2096
英文名称： P2 Primary Cell solution (1x 96)
型号:null    产品货号： VSOP-2096
价格:请致电：010-57128832,18610462672
品牌: 德国进口

北京泽平代理的Lonza Nucleofector 细胞核转染仪 (原名Amaxa Nucleofector)，通过电穿孔技术，配合专用电转染试剂盒，能将DNA、RNA等物质导入细胞，并直接穿过核膜进入细胞核，实现高效转染。北京泽平是Lonza中国区一级代理，销售Lonza旗下全系列产品，如Lonza Nucleofector细胞核转染系统、CytoSMART活细胞成像系统、原代细胞和培养基、基础细胞培养基（DMEM、MEM、RPMI 1640）、内毒素检测试剂盒、支原体检测试剂盒及清除试剂盒、琼脂糖和闪胶等。自2009年起北京泽平成为Lonza全国代理，凭借优异的产品和服务，受到广大客户的认可！欢迎来电咨询北京泽平官方客服400-686-0100或13910005869或13521452266

erted-space"> Primary Macrophages is pre-optimized for transfecting primary macrophages. Macrophages are white blood cells within tissues, produced by the differentiation of monocytes. Monocytes and macrophages are phagocytes, acting in both non-specific defense (innate immunity) as well as to help initiate specific defense mechanisms (adaptive immunity) of vertebrate animals. Their role is to phagocytose (engulf and then digest) cellular debris and pathogens either as stationary or as mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen. They can be identified by specific expression of a number of proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), Lysozyme M, MAC-1/MAC-3 and CD68 by flow cytometry or immunohistochemical staining.  GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

 

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)


~70%
PRMI with 10% FBS
3:1
1xGenJet™ Transfection Buffer
15 minutes at RT
Yes
No
48 hours


EGFP
pEGFP-N3 (CMV promoter) 
75%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet & Protocol.pdf