预优化DNA转染试剂-Huh-7 英文名称: GenJet™ In Vitro DNA Tranfection Reagent for Huh-7 Cells | ||
型号:null 产品货号: SL100489-HUH | ||
价格:请致电:010-57128832,18610462672 | ||
品牌: 美国 | ||
Description GenJet™ DNA In Vitro Tranfection Reagent for HuH-7 Cells is pre-optimized for human HuH-7 cell transfection. HuH-7, derived from human cancer liver, is very hard to transfect. With our pripariotary peptide conjugation technology, this reagent was formulated to transfect HuH-7 cells with up to 70% efficiency (GFP positive cells). Refer to the following optimal transfection conditions for maximal transfection efficiency on HuH-7 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer A Picture Showing Transfection Efficiency of GenJet™ Reagent on HuH-7 Cells ![]() GenJet™ reagent is optimized for HuH-7 cells with exceptional efficiency in comparison of Lipofectamine 2000 (L2K) and Fugene HD. HuH-7 cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 48 hours post transfection Right Panel: Comparison of transfection efficiency of GenJet with Lipofecatmine 2000 (L2K), and Fugene HD on HuH-7 cells. GFP DNA (pEGFP-N3) was introduced to HuH-7 cells with different transfection reagents per manufacturers protocols. GFP positive cell (%) and fluorescence intensity were detected by passing through FACS 48 hours post transfection Left Panel: presence of serum and antibiotics enhances GenJet efficiency on HuH-7 cells. HuH-7 cell (grown on collagen treated dishes) was transfected with three different conditions-------serum and antibiotics free, presence of 10% serum and antibiotics followed by removal 5 hours post transfection and presence of 10% serum and antibiotics without removal 5 hours post transfection. ![]() GenJet™ reagent is optimized for HuH-7 cells. HuH-7 cells were grown per ATCC recommended culture medium on a collagen type I treated culture dish and transfected with GFP cDNA (pEGFP-N3) by GenJet™. The efficiency was checked 24 hours post transfection by Nikon Elipse 2000 confocal microscopy ![]() |