预优化DNA转染试剂-HELA
英文名称: GenJet™ In Vitro DNA Tranfection Reagent for Hela Cells
型号:null    产品货号: SL100489-HELA
价格:请致电:010-57128832,18610462672
品牌: 美国

 

Description
GenJet™ DNA In Vitro Tranfection Reagent for Hela is pre-optimized for Hela cell transfection. Refer to the following optimal transfection conditions for maximal transfection efficiency on Hela cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium 5 Hours After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)

~80%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes
48 hours


EGFP
pEGFP-N3 (CMV promoter) 

88%
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

A Picture Showing Transfection Efficiency of GenJet™ Reagent on Hela Cells

GenJet™ reagent is optimized for Hela cell (ATCC # CCL-2.2). Hela cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 48 hours post transfection
Data Sheet.pdf

宋体;mso-bidi-font-family:宋体;mso-font-kerning:0pt;mso-bidi-language:AR">札如病毒(SV)核酸检测试剂盒(PCR-荧光探针法)工作步骤

标准的PCR过程分为三步:

1.DNA变性(90-96℃):双链DNA模板在热作用下,氢键断裂,形成单链DNA

2.退火(复性)(25-65℃):系统温度降低,引物与DNA模板结合,形成局部双链。

3.延伸(68-75℃):在Taq酶(在72℃左右最佳的活性)的作用下,以dNTP为原料,从引物的5′端→3′ 端延伸,合成与模板互补的DNA链。

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