תESPES»ùÒòÖ²Îï PCR Mix
Ó¢ÎÄÃû³Æ£º ×ªESPES»ùÒòÖ²Îï PCR Mix
ÐͺÅ:null    ²úÆ·»õºÅ£º 131129L
¼Û¸ñ:ÇëÖµ磺010-57128832,18610462672
Æ·ÅÆ: Öйú
ÊÔ¼Á¼¶±ð£º ·Ö×ÓÉúÎïѧ¼¶

  

תESPES»ùÒòÖ²Îï PCR Mix½á¹û·ÖÎö£º

PCR²úÎïÄý½ºµçÓ¾£¬PCR·´Ó¦¹Üµ¥Ïò´«µÝÖÁÁíÉèÒ»¼äרÃŶÀÁ¢µÄµçӾʵÑéÊÒ£¬¸ù¾ÝƬ¶Î´óС¾­1.0-2.0%ÇíÖ¬ÌÇÄý½ºµçÓ¾·ÖÎö£¬µçÓ¾»º³åÒºÖмÓÈëÊÊÁ¿Ç׺ÏËØ±ê¼ÇµÄÇíÖ¬ÌÇÄý½º¿ÅÁ££¬ÔÚ×ÏÍâϹ۲ì½á¹û£¬Èô³öÏÖÓëÑôÐÔ¶ÔÕÕÏàͬµÄÌõ´ø¼´Îª¸Ãϸ¾úºÍ²¡¶¾ÑôÐÔ£¬¶øÒõÐÔ¶ÔÕÕûÓÐÌØÒìÐÔÀ©ÔöÌõ´ø¡£

תESPES»ùÒòÖ²Îï PCR Mix·´Ó¦£º

dH2O                                         37.5μl

5×Taq¾ÛºÏøbuffer (°üº¬ÒýÎï)                  10μl

Taq¾ÛºÏø                                    0.5μl

10mM dNTP                                    1μl

Ñù±¾Ãþ°å»ò¶ÔÕÕDNA                             1μl

Up  to  50μl

תESPES»ùÒòÖ²Îï PCR Mix×¢ÒâÊÂÏ

¢Ù ¿ªÊ¼¼ì²âǰÇë×ÐϸÔĶÁ¸Ã²úÆ·¼ò½éÈ«ÎÄ¡£

¢Ú²Ù×÷ʱӦ¾¡Á¿±ÜÃâ˵»°£¬ÒÔÃâÒýÆðÑùÆ·ÎÛȾ£¬¶øÔì³É¼ÙÑôÐÔ¡£

¢Û Õû¸ö¼ì²â¹ý³ÌÖУ¬·´Ó¦ÌåϵµÄÅäÖÆ¡¢Ñù±¾´¦Àí¼°¼ÓÑù¡¢PCRÀ©ÔöÓ¦·ÖÇøÓò½øÐУ¬ÒÔ±ÜÃâ½»²æÎÛȾ¡£

¢Ü ¾¡Á¿Ê¹ÓÃÒ»´ÎÐÔ²ÄÁÏ£¬°üÀ¨¹ýÂËоÎüÍ·£¬EPÊԹܣ¬Ïð½ºÊÖÌ׵ȡ£

¢Ý ʵÑéʱ£¬ÊÔ¼ÁºÐ×é³ÉÖеÄÊÔ¼Á³ýøÍâʹÓÃǰӦÖÃ4¡æÌáǰÈÚ»¯£¨»ìÔÈʱ½ûÖ¹¼¤ÁÒÕñµ´£©£¬Ã¸Ê¼ÖÕÖÃÓÚ-20¡æ±£´æ¡£

¢Þ ¼ì²â·´Ó¦¹ÜÖмÓÈëËùÓеÄÊÔ¼Áºó£¬Ó¦¾¡¿ìÉÏPCRÒǽøÐÐÀ©Ôö£¬ÒÔÃâÀ©ÔöÀäÆô¶¯¼°Ðγɹý¶àµÄ¶þ¾ÛÌå¡£

¢ß±£´æ:£­20 ¡æ, ±£ÖÊÆÚÒ»Äê¡£

תESPES»ùÒòÖ²Îï PCR Mix²úƷϵÁУºXY71207 RNAÏ´ÍÑÒº 50mL

µ°°×ÖÊÑо¿

XY80807¶¯Îïϸ°ûÁѽâÒº50mL(A)|50mL(B)XY131006RIPAÁѽâÒº(Ç¿)100mLXY131007RIPAÁѽâÒº(ÖÐ)100mLXY131008RIPAÁѽâÒº(Èõ)100mLXY131009NP-40ÁѽâÒº100mLXY90707¶¯Ö²Îï×ܵ°°×΢Á¿ÌáÈ¡ÊÔ¼ÁºÐ50´ÎXY91204¶¯Ö²ÎïĤµ°°×΢Á¿ÖƱ¸ÊÔ¼ÁºÐ50´ÎXY90613¶¯Îïϸ°ûºËµ°°×΢Á¿ÖƱ¸ÊÔ¼ÁºÐ25´Î|100´ÎXY91203Ò»²½Ê½°û½¬µ°°×΢Á¿ÖƱ¸ÊÔ¼ÁºÐ30´Î|100´ÎXY121208ÒºÌåÑùÆ·µ°°×´¿»¯»ØÊÕÊÔ¼ÁºÐ50´ÎXY81218Ö²Îï×ܵ°°×ÖÊ΢Á¿ÌáÈ¡ÊÔ¼ÁºÐ(SDS-PAGE)30´ÎXY81219Ö²Îï×ܵ°°×ÖÊ΢Á¿ÌáÈ¡ÊÔ¼Á(2DE)30´Î|100´ÎXY81202Õæ¾ú×ܵ°°×ÖÊ΢Á¿ÌáÈ¡ÊÔ¼ÁºÐ50´ÎXY90902ϸ¾ú×ܵ°°×ÖÊ΢Á¿ÌáÈ¡ÊÔ¼ÁºÐ30´ÎXY100929ϸ¾úĤµ°°×ÖÊ΢Á¿ÌáÈ¡ÊÔ¼ÁºÐ50´ÎXY131064ϸ°û±íÃæµ°°×·ÖÀëÊÔ¼ÁºÐ8´ÎXY131066***Ëữµ°°×¸»¼¯ÊÔ¼ÁºÐ10´ÎXY131067ConA Ìǵ°°×·ÖÀëÊÔ¼ÁºÐ10´ÎXY131205WGA Ìǵ°°×·ÖÀëÊÔ¼ÁºÐ10´ÎXY90508°üº­Ìå΢Á¿´¿»¯ÊÔ¼ÁºÐ20´ÎXY90509°üº­ÌåÖÐÁ¿´¿»¯ÊÔ¼ÁºÐ5´ÎXY90510°üº­Ìå´óÁ¿´¿»¯ÊÔ¼ÁºÐ2´ÎXY131121µ°°×ÕÛµþÊÔ¼ÁºÐ100´ÎXY100302°üº­Ìåµ°°×Èܽ⼰¸´ÐÔÌ××°100´ÎXY100102һվʽHis±ê¼Çµ°°×ÖÊ΢Á¿´¿»¯Ì××°20´Î(A)|20´Î(B)XY100101ÄøÖù½éÖÊ2mL|10mLXY131057îÜÖù½éÖÊ10mLXY120801ÄøÖùԭλÔÙÉúÊÔ¼ÁºÐ20´ÎXY100202Íÿ¹His±êÇ©¶à¿¹£¬HRP±ê¼Ç100μLXY111116һվʽGST±ê¼Çµ°°×ÖÊ΢Á¿´¿»¯Ì××°20´Î(A)|20´Î(B)XY120101¹Èë׸ÊëÄÇíÖ¬ÌÇ2mLXY120705L-¹Èë׸ÊëÄ(»¹Ô­ÐÍ)5gXY120534Íÿ¹GST±êÇ©¶à¿¹20μLXY120535HRP±ê¼ÇµÄÍÿ¹GST±êÇ©¶à¿¹20μLXY130871һվʽMBP±êÇ©µ°°×´¿»¯Ì××°20´ÎXY130573СÊó¿¹His±êÇ©µ¥¿¹100μLXY130574СÊó¿¹Flag±êÇ©µ¥¿¹100μLXY130575СÊó¿¹c-Myc±êÇ©µ¥¿¹100μLXY130576СÊó¿¹HA±êÇ©µ¥¿¹100μLXY130577СÊó¿¹GST±êÇ©µ¥¿¹100μLXY130578СÊó¿¹V5±êÇ©µ¥¿¹100μLXY130579СÊó¿¹MBP±êÇ©µ¥¿¹100μLXY130580СÊó¿¹GFP±êÇ©µ¥¿¹100μLXY130581СÊó¿¹mCherry±êÇ©µ¥¿¹100μLXY130582СÊóRFP±êÇ©µ¥¿¹100μLXY130583СÊó¿¹VSV-G±êÇ©µ¥¿¹100μLXY130584СÊó¿¹T7±êÇ©µ¥¿¹100μLXY130585СÊó¿¹CBP±êÇ©µ¥¿¹100μLXY130586СÊó¿¹HSV±êÇ©µ¥¿¹100μLXY130587СÊó¿¹E2±êÇ©µ¥¿¹100μLXY130588СÊó¿¹S1±êÇ©µ¥¿¹100μLXY130589СÊó¿¹Tag-100±êÇ©µ°°×µ¥¿¹100μLXY130590СÊó¿¹HPC4±êÇ©µ°°×µ¥¿¹100μLXY130591СÊó¿¹SUMO±êÇ©µ°°×µ¥¿¹100μLXY130592HRP±ê¼ÇÄڲο¹Ìå30μLXY131202¼´ÓÃÐÍGFP±êÇ©µ°°×ÁѽâÒº100uLXY130413¸ÎËØÇíÖ¬ÌÇ5mL|50mLXY130417À¶½ºÇíÖ¬ÌÇ5mL|50mLXY130421DEAE½»»»½éÖÊ50mLXY130423Q½»»»½éÖÊ50mLXY130422CM½»»»½éÖÊ50mLXY130424SP½»»»½éÖÊ50mLXY131020Sephacryl S-400»ùÖÊ10mLXY130425***»ù½éÖÊ50mLXY130426¶¡»ù½éÖÊ50mLXY130427¶¡»ùÁò½éÖÊ50mLXY130418Protein AÇíÖ¬ÌÇ1mLXY130420Protein GÇíÖ¬ÌÇ1mLXY131201Protein A+GÇíÖ¬ÌÇ2mLXY100939¸£ÁÖ·ÓÊÔ¼Á100mLXY90610ÂѰ׵°°×±ê׼Ʒ1mLXY131070ţѪÇå°×µ°°×±ê׼Ʒ1mLXY131071ţѪÇåγ Çòµ°°×±ê׼Ʒ1mLXY131072ԤϡÊÍBSA µ°°×±ê׼Ʒ1mLXY131220ԤϡÊÍBGG µ°°×±ê׼Ʒ1mLXY131074SDS-PAGE ÑùÆ·Ô¤´¦ÀíÊÔ¼ÁºÐ50´ÎXY131122¿¼Âí˹ÁÁÀ¶R-25010gXY131123¿¼Âí˹ÁÁÀ¶G-25010gXY131078ÌìÈ»µ°°×A1mgXY131079ÖØ×éµ°°×A1mgXY131080ÖØ×éµ°°×G1mgXY131081ÖØ×éµ°°×A/G1mgXY131082ÖØ×éµ°°×L1mgXY131075Ìǵ°°×ȾɫÊÔ¼ÁºÐ10´ÎXY131076***Ëáµ°°×ȾɫÊÔ¼ÁºÐ10´ÎXY131077×é°±Ëá±êÇ©µ°°×ȾɫÊÔ¼ÁºÐ10´ÎXY80814Bradford·¨µ°°×¶¨Á¿ÊÔ¼ÁºÐ100mL|200mLXY80815BCA·¨µ°°×¶¨Á¿ÊÔ¼ÁºÐ500´ÎXY80816³¬ÃôÐÍBCA·¨µ°°×¶¨Á¿ÊÔ¼ÁºÐ500´ÎXY131069»¹Ô­¼Á¼æÈÝÐÍBCAµ°°×¶¨Á¿ÊÔ¼ÁºÐ250´ÎXY101102¸ÄÁ¼Lowry·¨µ°°×¶¨Á¿ÊÔ¼ÁºÐ1000´ÎXY80808µ°°×øÒÖÖÆ¼Á»ìºÏÒº2×0.5mLXY80809µ°***ËáøÒÖÖÆ¼Á»ìºÏÒº11mLXY130525µ°***ËáøÒÖÖÆ¼Á»ìºÏÒº25mLXY130526ϸ¾úרÓõ°°×øÒÖÖÆ¼Á10mLXY130527²¸È鶯ÎïרÓõ°°×øÒÖÖÆ¼Á1mLXY130528Ö²ÎïרÓõ°°×øÒÖÖÆ¼Á1mLXY130529½ÍĸרÓõ°°×øÒÖÖÆ¼Á5mLXY130530His±êÇ©µ°°×רÓõ°°×øÒÖÖÆ¼Á10mLXY130531×éÖ¯ÅàÑø×¨Óõ°°×øÒÖÖÆ¼Á1mLXY130543α2¾ÞÇòµ°°×25Inh.UXY130544AEBSFÈÜÒº5mLXY130534¿¹ÄýѪø¢óÈÜÒº1mLXY130535APMSFÈÜÒº1mLXY100834Òȵ°°×øÒÖÖÆ¼ÁÈÜÒº10mLXY130536ÒÖ°±ëÄøÈÜÒº5mLXY130537¸Æµ°°×øÒÖÖÆ¼Á¢ñÈÜÒº10mLXY100538¸Æµ°°×øÒÖÖÆ¼Á¢òÈÜÒº10mLXY100539ÒÈÄýÈéµ°°×øÒÖÖÆ¼ÁÈÜÒº1mLXY130540¶þÂÈÒì***ÈÜÒº10mLXY130541E-64µ°°×øÒÖÖÆ¼ÁÈÜÒº10mLXY100837EDTAÈÜÒº,0.5M,µ°°×¼¶10mLXY130532EGTAÈÜÒº10mLXY130542Ë®òÎËØÈÜÒº2000ATUXY100836ÁÁÒÖµ°°×øëÄÈÜÒº1mLXY130533ľ¹Ïµ°°×øÒÖÖÆ¼ÁÈÜÒº10mLXY100835θµ°°×øÒÖÖÆ¼ÁÈÜÒº1mLXY130545ì¢õ£¶þëÄÈÜÒº10mLXY100833PMSFÈÜÒº5mLXY131043CHAPS5gXY131044ÐÁ¼×»ùβ ÆÏÌÇÜÕ1gXY131045ÐÁ¼×»ùÁòßÁà«ÆÏÌÑÌÇÜÕ5gXY131046n-Ê®¶þÍé»ù-β-D-ÂóÑ¿ÌÇÜÕ1gXY131047ÍÑÑõµ¨ËáÄÆ5gXY131048µ¨ËáÄÆ5gXY131049Ê®¶þÍé»ù»ÇËáÄÆ100gXY131050È¥¹¸¼ÁÈ¥³ýÊ÷Ö¬10mLXY131051Ê®¶þÍé»ù»ÇËáÄÆÇå³ý¼Á25mLXY131052β- ***£¬µ°°×¼¶10mLXY131053µ°°×¼¶DTT5gXY131054Ãâ³ÆÁ¿µ°°×¼¶DTT48¹ÜXY131055TCEP ÑÎËáì¢1gXY131056TCEP ÈÜÒº5mLXY131120ÑÎËáëÒÈÜÒº200mLXY131222ßäßòÈÜÒº100mLXY131058ßäßò25gXY131059Ñǰ±¶þÒÒËá½éÖÊ10mLXY131061¹Èë׸ÊëÄS ×ªÒÆÃ¸1mgXY81216ºËËáÇå³ý¼Á1.5mLXY81029·Ç±äÐÔ·¨µ°°×³ÁµíÊÔ¼ÁºÐ10´ÎXY81217΢Á¿µ°°×³ÁµíÊÔ¼ÁºÐ50´ÎXY61205ѪÇå°×µ°°×Çå³ý¼Á15´ÎXY100915SDS-PAGEÔ¤ÖÆ½º12¿éXY80810һվʽSDS-PAGEµçÓ¾Ì××°30´ÎXY100867SDS-PAGEŨËõ½ºÅ佺Һ500mLXY100868SDS-PAGE·ÖÀ뽺Å佺Һ500mLXY81204SDS-PAGEÉÏÑùÒº10mLXY81203SDS-PAGEµçÓ¾Òº5L|20LXY100908һվʽ³¤Ð§ÆÚSDS-PAGEµçÓ¾Ì××°30´Î(A)|30´Î(B)XY100909³¤Ð§ÆÚSDS-PAGEÅ佺Һ200mLXY100910³¤Ð§ÆÚSDS-PAGE»¹Ô­¼Á10mLXY100911³¤Ð§ÆÚSDS-PAGEÉÏÑùÒº1mLXY100912³¤Ð§ÆÚSDS-PAGEµçÓ¾Òº(¸É·Û)20L(A)XY81205һվʽTricine-SDS-PAGEÌ××°30´ÎXY81225Tricine-SDS-PAGEÅ佺Һ250mL|1000mLXY81213Tricine-SDS-PAGEÉÏÑùÒº1mL|5mLXY81214Tricine-SDS-PAGEÑô¼«µçÓ¾Òº(¸É·Û)10L|50LXY81226Tricine-SDS-PAGEÒõ¼«µçÓ¾Òº(¸É·Û)10L|50LXY81212һվʽµ°°×·Ç±äÐÔPAGEÌ××°30´Î(A)|30´Î(B)|30´Î(C)XY100828¸ßpH»º³åÒºÌ××°30´ÎXY100829ÖÐpH»º³åÒºÌ××°30´ÎXY100830µÍpH»º³åÒºÌ××°30´ÎXY70102µ°°×ÖʵçÓ¾·Ö×ÓÁ¿±ê×¼15´Î(A)|20´Î(B)|10´Î(C)XY70704ԤȾµ°°×ÖʵçÓ¾·Ö×ÓÁ¿±ê×¼10´Î(A)|10´Î(B)XY90402µ°°×½ºÎ¢Á¿»ØÊÕÊÔ¼ÁºÐ50´ÎXY90403µ°°×½ºÖÐÁ¿»ØÊÕÊÔ¼ÁºÐ5´ÎXY80812¿¼Âí˹ÁÁÀ¶È¾Òº250mLXY61204Ò»²½Ê½PAGEȾҺ10´ÎXY111203ÂÌÈçÀ¶µ°°×ȾҺ250´ÎXY100810³¬¿ìµ°°×ÒøÈ¾ÊÔ¼ÁºÐ1000mLXY100811ÖÊÆ×¼æÈÝÐ͵°°×ÒøÈ¾ÊÔ¼ÁºÐ10´ÎXY100603ÒøÈ¾Çå³ý¼Á30´ÎXY81215һվʽWesternÓ¡¼£Ì××°(ÏÔÉ«·¨)20´Î(A)|20´Î(B)XY131104Western ·â¶ÂÒº500mLXY131105ÀÒµ°°×·â¶ÂÒº100mL(A)|100mL(B)XY131236ţѪÇå°×µ°°×·â¶ÂÒº250mLXY131109Tris »º³åÑÎÈÜÒº(TBS)10°üXY131112ÂÈÝÁ·ÓÈÜÒº50mLXY131114ABTS ÈÜÒº50mlXY131115OPD ¸É·Û25gXY131116pNPP ¸É·Û1gXY131108ONPG ¸É·Û1gXY101122ÄáÁúĤ1ÕÅXY101123PVDFĤ1ÕÅXY81206°±»ùºÚȾĤҺ250mLXY100924Àö´ººìSȾĤҺ250mLXY100928ʪ·¨µçתҺ(¸É·Û)20LXY81207°ë¸É·¨µçתҺ(¸É·Û)20LXY100878WesternÓ¡¼£Ä¤·âĤҺ100mLXY81208Ó¡¼£Ä¤¿¹ÌåÏ¡ÊÍÒº100mLXY80813WesternÓ¡¼£Ä¤¿¹ÌåÇå³ý¼Á50mL|200mLXY1008315-äå-4-ÂÈ-3-ßÅßá***Ëá100mgXY100832ËĵªßòÀ¶250mgXY81224BCIP/NBTÏÔÉ«ÊÔ¼ÁºÐ100mLXY100214Ô¤»ìÐÍBCIP/NBTÈÜÒº100mLXY81223ÔöÇ¿ÐÍDABÏÔÉ«ÊÔ¼ÁºÐ100mLXY101106³ÁµíÐÍTMBÏÔÉ«ÊÔ¼ÁºÐ100mLXY91101Ò»¹Üʽ TMB ÏÔɫҺ100mL(A)XY120201³¬Ãô»¯Ñ§·¢¹â(HRP)¼ì²âÊÔ¼ÁºÐ25mL|250mLXY131192³¬Ãô»¯Ñ§·¢¹â(AP)¼ì²âÊÔ¼ÁºÐ25mLXY131142³Ã×ŵ1gXY131143AMPPD100mgXY131118ÒÒÏ©ÒÒ¶þ´¼200mLXY131119HRP Îȶ¨¼Á/ Ï¡ÊͼÁ200mLXY130565β-ActinСÊ󵥿¹30μLXY130566α-ActinСÊ󵥿¹30μLXY130567β-TubulinСÊ󵥿¹30μLXY130568α-TubulinСÊ󵥿¹30μLXY130569GAPDHСÊ󵥿¹30μLXY130570Ö²ÎïActinСÊ󵥿¹30μLXY130571×éµ°°×3.1СÊ󵥿¹30μLXY130572COX IVÍõ¥¿¹20μLXY131144ɽÑò¿¹Ð¡ÊóIgG(H+L),¼îÐÔ***Ëáø±ê¼Ç100μLXY131145ɽÑò¿¹ÍÃIgG(Fc)£¬¼îÐÔ***Ëáø±ê¼Ç100μLXY131146ɽÑò¿¹ÈËIgG(H+L)£¬¼îÐÔ***Ëáø±ê¼Ç100μLXY131147ɽÑò¿¹´óÊóIgG(H+L)£¬¼îÐÔ***Ëáø±ê¼Ç100μLXY131148¿¿¹É½ÑòIgG£¬¼îÐÔ***Ëáø±ê¼Ç60μLXY131149ɽÑò¿¹ÍÃIgG(H+L)£¬À±¸ù¹ýÑõ»¯Îïø±ê¼Ç300μLXY131150ɽÑò¿¹Ð¡ÊóIgG(H+L)£¬À±¸ù¹ýÑõ»¯Îïø±ê¼Ç300μLXY131151ɽÑò¿¹ÈËIgG(H+L)£¬À±¸ù¹ýÑõ»¯Îïø±ê¼Ç300μLXY131152Íÿ¹¼¦IgY£¬À±¸ù¹ýÑõ»¯Îïø±ê¼Ç300μLXY131153¿¿¹É½ÑòIgG£¬À±¸ù¹ýÑõ»¯Îïø±ê¼Ç60μLXY131154ɽÑò¿¹Ð¡ÊóIgG£¬Ó«¹âËØ488±ê¼Ç0.1mgXY131155ɽÑò¿¹Ð¡ÊóIgG£¬Ó«¹âËØ555±ê¼Ç0.1mgXY131156ɽÑò¿¹ÍÃIgG£¬ÒìÁò*ËáÓ«¹âËØ±ê¼Ç0.1mgXY131157Ôåºìµ°°×±ê¼ÇɽÑò¿¹ÍÃIgG0.1mgXY131158Ó«¹âËØ488±ê¼ÇɽÑò¿¹ÍÃIgG0.1mgXY131159Ó«¹âËØ555±ê¼ÇɽÑò¿¹ÍÃIgG0.1mgXY131160ÒìÁò*ËáÓ«¹âËØ±ê¼ÇɽÑò¿¹Ð¡ÊóIgG0.1mgXY131161ɽÑò¿¹Ð¡ÊóIgG£¬Ôåºìµ°°×±ê¼Ç0.1mgXY100841β-***1.5mLXY100881SDSÈÜÒº(10%,µçÓ¾¼¶)100mLXY90311µçÓ¾¼¶Tricine¸É·Û100gXY111007MALDIµ°°×ÑùÆ·´¦ÀíÊÔ¼ÁºÐ500´Î

¹¤¾ßø