英文名称： GQH-HPB
试剂级别： 试剂级
人乙型肝炎定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ Human Hepatitis B qPCR Array Kit (GQH-HPB) is designed to facilitate gene expression profiling of 88 key genes involved in hepatitis B pathogenesis. Hepatitis B is a liver infectious disease caused by the Hepatitis B virus (HBV). The HBV infection of hepatocytes triggers immune response cascades to regulate HBV replication and hepatocyte survival. Brief examples of how included genes may be grouped are shown below:
- HBV binding factor: HSPG2, CPD
- Signaling pathways
- MAPK: MAPK1, MAP3K1, MAPK8, MAPK10, MAP2K1, RAF1, ELK1, YWHAB, YWHAQ, FOS
- PI3K/AKT: AKT1, PIK3CA, PIK3CG, PIK3R1, PIK3R3, PIK3R5
- Toll-like receptor: TLR2, TLR3, TLR4, IFNAs, IFNAR1, IFNB1
- JAK/STAT: STAT1, STAT3, JAK1, MMP9, NFKB1, RELA, SRC
- Genes implicated in HBV infection: LAMTOR5, RSF1, APOBEC3G, GPT, TNF, AIP, AFP, MBL2, F2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Hepatitis B qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | AFP | ATP6AP1 | DDX58 | HSPG2 | IFNA2 | IFNB1 | MAP2K4 | MRC1 | PTMA | SRC | TIRAP | β-actin |
| B | AIP | CPD | EGR3 | IFIH1 | IFNA21 | IFNG | MAP3K1 | NFKB1 | RAF1 | STAT1 | TLR2 | GAPDH |
| C | AKT1 | CREB3 | ELK1 | IFNA1 | IFNA4 | IKBKE | MAPK1 | PIK3CA | RARB | STAT3 | TLR3 | LDHA |
| D | ALB | CREB3L1 | F2 | IFNA10 | IFNA5 | IRF3 | MAPK10 | PIK3CG | RELA | STAT6 | TLR4 | NONO |
| E | APOBEC3G | CREB3L2 | FGL2 | IFNA13 | IFNA6 | JAK1 | MAPK8 | PIK3R1 | RFX1 | TGFB1 | TNF | PPIH |
| F | ASGR1 | CTLA4 | FOS | IFNA14 | IFNA7 | LAMTOR5 | MAVS | PIK3R3 | RSF1 | TGFBR1 | VDAC3 | GDC |
| G | ATF4 | DDB2 | GGT1 | IFNA16 | IFNA8 | MAP2K1 | MBL2 | PIK3R5 | SLC17A5 | TICAM1 | YWHAB | PPC |
| H | ATF6B | DDX3X | GPT | IFNA17 | IFNAR1 | MAP2K2 | MMP9 | PSMA7 | SON | TICAM2 | YWHAQ | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK043 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-HPB |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
近几年的新药研发成功率在逐年下降，其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%
人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许
多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利
用这些原代细胞株所甄别和筛选出来的候选药物，其诊治人类疾病的成功几率将大大增加。这样不但大大
节省了新药的开发成本，而且将极大地提高人类的健康质量。这些产品从根本上提高了全球生命医学研究、
人类重要疾病药物研发、新药研发的成功率。
所以上海中乔新舟生物科技有限公司致力于提供优质原代细胞产品和完善的售后服务。
Sciencell公司部分原代细胞目录（如需要其他细胞资料请发邮件至 wwwfudan@163.com索取）：
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技术服务、论文相关服务、采购外包等整体服务，目前已经成长为国内领先的转化医学外包品牌。
中乔新舟的PI团队已经发展到专职PI 20人，联席PI 312人，其中大多数拥有海外背景。截止到2015年以PI或联席PI为
第一作者发表SCI 论文1682 篇（总IF 值为5721.82，其中影响因子IF>5.0 的论文216 篇，IF>10.0论文32 篇）。
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