英文名称： GQH-DDS
人感知DNA损伤定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ Human DNA Damage Sensing qPCR Array Kit (GQH-DDS) is designed to facilitate gene expression profiling of 40 key genes involved in DNA damage sensing. DNA damages, such as mutations, breaks, cross-links, deletions/insertions and translocations, are constantly generated due to internal and external stresses. Appropriate DNA damage responses must be implemented to maintain genome stability. Brief examples of how included genes may be grouped are shown below:
- DNA damage sensors: ATM, ATR, PRKDC, PARP1
- Cell cycle regulation: CHEK1, CHEK2, CCNB1, CCNB2, CCNB3, CCND1, CCND2, CCND3, CCNE2, CDK2, CDK4, CDK6
- ATM signaling pathway: ABL1, BRCA1, CDC25A, CDKN1A, CHEK1, CHEK2, GADD45A, RAD51, RBBP8, TP53
- ATR signaling pathway: CDC25A, CDC25C, CDK2, CHEK1, CHEK2, HUS1, MDM2, RAD1, RAD17, RAD9A, TP53
- Apoptosis: ABL1, ATM, BAX, BRCA1, CASP3, CASP9, CDKN1A, CHEK2, PRKDC, RAD91, TP53
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human DNA Damage Sensing qPCR Array Plate Layout*
(8 controls in Bold and Italic)
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| 1 | 2 | 3 | 4 | 5 | 6 | 7 | erning:0pt;mso-bidi-language:AR">工作步骤 标准的PCR过程分为三步： 1.DNA变性（90℃-96℃）：双链DNA模板在热作用下，氢键断裂，形成单链DNA 2.退火（复性）（25℃-65℃）：系统温度降低，引物与DNA模板结合，形成局部双链。 3.延伸（68℃-75℃）：在Taq酶（在72℃左右最佳的活性）的作用下，以dNTP为原料，从引物的5′端→3′ 端延伸，合成与模板互补的DNA链。 古典猪瘟病毒PCR检测试剂盒 产品特点：快速，灵敏，特异，准确，安全，简单 HZ-169 古细菌+真细菌种属鉴定PCR Mix 3 产品特点：快速，灵敏，特异，准确，安全，简单 HZ-170 古细菌种属鉴定PCR Mix 1 产品特点：快速，灵敏，特异，准确，安全，简单 HZ-171 冠状病毒(229E/NL63)PCR检测试剂盒 产品特点：快速，灵敏，特异，准确，安全，简单 HZ-172 冠状病毒(OC43/HKU1)PCR检测试剂盒 产品特点：快速，灵敏，特异，准确，安全，简单 HZ-173 汉坦病毒PCR检测试剂盒 产品特点：快速，灵敏，特异，准确，安全，简单 HZ-174 汉坦病毒Ⅰ型PCR检测试剂盒 产品特点：快速，灵敏，特异，准确，安全，简单 HZ-175 汉坦病毒Ⅱ型PCR检测试剂盒 产品特点：快速，灵敏，特异，准确，安全，简单 HZ-176 |