英文名称： GQH-NTM
人神经传导与膜转运定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ human neural transmission and membrane trafficking qPCR array kit (GQH-NTM) is designed to facilitate gene expression profiling of key genes involved in (i) human neurotransmission; (ii) membrance trafficking; and (iii) related diseases such as generalized anxiety disorder, DDC deficiency, TH deficiency, and SPR deficiency. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Neural Transmission and Membrane Trafficking qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | ADRA1A | BDNF | CHRNA5 | DRD2 | GABRA2 | GABRG3 | GRIK1 | GRM3 | HTR2A | OPTN | SNAP25 | β-actin |
| B | ADRA1D | BTD | CHRNA6 | DRD4 | GABRA4 | GABRQ | GRIK2 | GRM4 | HTR2C | OTC | SOD1 | GAPDH |
| C | ADRA2A | CHMP2B | CHRNA7 | DRD5 | GABRA5 | GABRR2 | GRIK4 | GRM6 | HTR3A | PTS | SPR | LDHA |
| D | ADRB2 | CHRM1 | CHRNE | FIG4 | GABRB1 | GCH1 | GRIK5 | GRM7 | HTR4 | QDPR | TARDBP | NONO |
| E | ADRB3 | CHRM4 | COMT | FUS | GABRB3 | GH1 | GRIN2A | HTR1A | HTR7 | RAB10 | TH | PPIH |
| F | AKR1B1 | CHRM5 | DBH | G6PD | GABRE | GRIA1 | GRIN2B | HTR1B | LLGL1 | SLC6A2 | TPH1 | GDC |
| G | ALDH5A1 | CHRNA3 | DHFR | GABBR1 | GABRG1 | GRIA2 | GRIN2C | HTR1D | LPL | SLC6A3 | VAPB | PPC |
| H | ALS2 | CHRNA4 | DRD1 | GABBR2 | GABRG2 | GRIA3 | GRM1 | HTR1F | MAOA | SLC6A4 | VCP | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK008 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-NTM |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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