英文名称： GQH-TEK
试剂级别： 试剂级
人Tie2 / Angpt信号定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Kit (GQH-TEK) is designed to facilitate gene expression profiling of 88 key genes involved in Tie2 signaling transduction, which is a key component in the formation of blood vessels during angiogenesis. Brief examples of how included genes may be grouped according to function are shown below:
- Ligands & receptors: ANGPT1, ANGPT2, TEK, TIE1
- Morphology: GRB2, PTPN11, SOS1, PTK2, RHOA
- Motility: PLD1, MAP2K1, DOK2, NCK1, CRK
- Transcription factors: STAT1, STAT5B, MEF2A, FOXO3, EGR1
- Cell cycle regulation: CDC42, NFKB1, TNIP2, RIPK1, DIABLO
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | AFF1 | CCNT2 | DDX5 | FUS | HIST1H3D | HIST2H3D | MAX | MYC | PAX8 | SLC45A3 | TFE3 | β-actin | ||||
| B | ARNT2 | CDK9 | ELK4 | GOLPH3 | HIST1H3E | HIST3H3 | MDM2 | MYCN | PLAT | SMAD1 | TGFBR2 | GAPDH | ||||
| C | ASPSCR1 | CDKN1A | ERG | GOLPH3L | HIST1H3F | IGF1 | MEN1 | NFKB1 | PLAU | SP1 | TMPRSS2 | LDHA | ||||
| D | ATF1 | CDKN1B | ETV1 | H3F3B | HIST1H3G | IGF1R | MET | NFKBIZ | PPARG | SPINT1 | TP53 | NONO | ||||
| E | ATM | CEBPB | ETV4 | H3F3C | HIST1H3H | IL1R2 | MLF1 | NGFR | PRCC | SS18 | TSPAN7 | PPIH | ||||
| F | BAIAP3 | COMMD3 | EWSR1 | HIST1H3A | HIST1H3I | IL2RB | MLLT1 | NTRK1 | RXRA | SSX2 | WT1 | GDC | ||||
| G | BMI1 | CXCL8 | FEV | HIST1H3B | HIST1H3J | IL6 | MLLT3 | NUPR1 | RXRB | SSX2B | ZBTB17 | PPC | ||||
| H | CCNT1 | DDIT3 | FOXO1 | HIST1H3C | HIST2H3A | KMT2A | MMP9 | PAX7 | RXRG | TAF15 | ZEB1 | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK031 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-TMS |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | GQH-TMS is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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