英文名称： GQH-MEL
人黑色素瘤定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ human melanoma qPCR array kit (GQH-MEL) is designed to facilitate gene expression profiling of human melanoma related genes. Melanoma, also known as malignant melanoma, is a type of malignant cancer that results from uncontrolled growth of melanocytes, which are melanin-producing, pigmented cells. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery Human Melanoma qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | ABCC2 | BRAF | CTGF | EGFR | GNAQ | ITGA7 | MITF | OPN | PPP2R2C | SHOX2 | TM4SF13 | β-actin |
| B | AKT1 | BRCA2 | CTNNB1 | FABP7 | HOXB7 | ITGB3 | MLANA | MAPK14 | PTPRF | SNAI2 | TMEM229B | GAPDH |
| C | AKT2 | BRN2 | CXCR4 | FGF13 | HRAS | KIT | MX1 | PAX3 | Rab5b | SNAI1 | TNC | LDHA |
| D | AKT3 | CDH1 | CYR61 | FGFR2 | IGF1 | KRAS | Necdin | PIK3CA | RB1 | SOS1 | TP53 | NONO |
| E | ASXL1 | CDKN2A | DNAJA1 | FGFR3 | IGFBP3 | LIF | NOTCH2 | PIK3CB | RNF128 | SOX10 | TPD52 | PPIH |
| F | BCL2 | CHP2 | DTX3L | FOXD1 | ISG15 | MAPK3 | NPM2 | PIK3CD | RPS6KA1 | STK11 | TYRP1 | GDC |
| G | BCL2L1 | COL11A1 | EDNRB | FOXD3 | ITGA4 | MC1R | NRAS | PLA1A | RUNX3 | TIMP3 | UCHL1 | PPC |
| H | BMI | CSPG4 | ERCC2 | GAPDHS | ITGA6 | MCAM | OAS1 | PMEL | SHC4 | TM4SF1 | WNT4 | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK012 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-MEL |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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