ScienCells GeneQuery™ human melanocyte cell biology qPCR array kit (GQH-MCB) is designed to facilitate gene expression profiling of genes involved in melanocyte specific functions, and genes expressed at high levels in melanocyte compared to keratinocyte and fibroblast. 88 genes are selected in this kit based on public database and literature research.      

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

 

GeneQuery™ Human Melanocyte Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCC2

BHLHE41

CMPK2

EME1

FRMD5

ITPR1

MC1R

NR4A3

PPM1H

ROPN1

SOX10

β-actin

B

ADCK1

BMPR1B

CSPG4

EOMES

GJB1

KCNN2

MCAM

OCA2

PRDM7

RPS6KA1

THEM6

GAPDH

C

ADCY1

BRAF

CTTNBP2

ESR2

GNB2L1

KIT

MCF2

PKN3

PRKACA

RTP4

TLR1

LDHA

D

ADCY2

BST2

CYTL1

FABP7

GPR143

LEF1

MCOLN2

PKNOX2

PRKCB

RUNX3

TRIM6

NONO

E

ADCY3

CADM3

DNMT3A

FAM124A

HELZ2

LRRC45

MITF

PLA1A

QPCT

RXRG

TRP2

PPIH

F

ANKRD37

CD200

DPY19L2

FAXC

HOXB7

LSM11

MLANA

PLEKHH1

RAB20

SCG2

TSPAN10

GDC

G

ANO5

CDH19

EDNRB

FOXD3

HPDL

LYPD1

MMP8

PMEL

RNF157

SFMBT2

TYR

PPC

H

ARL9

CDKN2A

EGFL8

FOXRED2

HRAS

MAP2K1

NPM2

POMC

RNF182

SHC4

TYRP1

NTC

 

* gene selection may be updated based on new research and development