Oct4 (Octamer-binding protein 4) is a homeodomain transcription factor of the POU family, expressed in embryonic stem (ES) cells and embryonic carcinoma (EC) cells. ·Description Recombinant Human Oct4 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 360 amino acids and having a molecular mass of 38.6kDa. ·Quality Control Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Gly-His-Leu. Endotoxin:Less than 0.1ng/μg (1.0EU/μg) of rHuOct4. as determined by LAL test. ·Formulation SteriLyophilized from a 0.2um filtered protein solution in PBS. ·Reconstitution It is recommended to reconstitute the lyophilized rHuOct4 in sterile PBS not less than 100μg/ml, which can then be further diluted to other solutions. ·Storage Lyophilized rHuOct4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human Oct4 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples with 0.1% HSA or BSA are stable up to 3 months below -18°C for future use. Please avoid freeze-thaw cycles. |