| Cysteine-rich secretory protein (CRISP) 2, also known as TPX1, is a member of the CRISP subdivision of the CAP superfamily, which is defined by a CAP signature motif common to the entire superfamily, and has been localized to both the sperm acrosome and tail. Within mammals, four CRISPs have been defined: CRISP1 (acidic epididymal glycoprotein [AEG], proteins D and E), CRISP2 (TPX1), CRISP3, and CRISP4. All are strongly expressed within the male reproductive tract, suggesting key roles in male fertility. CRISP2 may regulate some ion channels activity and therebye regulate calcium fluxes during sperm capacitation.
·Description
Novoprotein Recombinant Human CRISP-2 produced by mammalian cells expression system.Target protein is expressed with sequence (Lys22-Tyr243) of Human crisp-2 (Uniprot #P16562) fused with a polyhistidine tag at the C-terminus.
·Quality Control
Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Endotoxin:Less than 0.1ng/μg (1EU/μg) of CRISP-2 (Human) ·Formulation
Novoprotein Recombinant Human CRISP2 was lyophilized at 1mg/ml in 20mM PBS. ·Reconstitution
We recommend a quick spin followed by reconstitution of Novoprotein Recombinant Human CRISP2 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to working solutions. ·Storage
Lyophilized Recombinant Human CRISP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human CRISP2 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use. Please avoid freeze-thaw cycles. | 