| 出品公司: | SIGMA |
|---|---|
| 载体名称: | pFLAG-CMV-5a |
| 质粒类型: | 哺乳动物表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 多克隆位点；限制性内切酶 |
| 启动子: | CMV |
| 载体大小: | 4.7 kb |
| 5 测序引物及序列: | CMV Forward：CGCAAATGGGCGGTAGGCGTG |
| 3 测序引物及序列: | -- |
| 载体标签: | FLAG tag (C-末端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | 常用细胞系，如293、Hela等 |
| 备注: | CMV启动子驱动目的蛋白高水平表达； FLAG标签含8个氨基酸残基（DYKDDDDK） 一般位于融合蛋白的外表面，具有高度免疫灵敏性，便于检测和纯化目的蛋白； |
| 产品目录号: | E7523 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
英文名称： pFLAG-CMV-5a载体哺乳细胞表达载体质粒图谱序列抗性说明书价格报价
试剂级别： 分子生物学级
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
Product DescriptionVersions of the pFLAG-CMV-5 expression vector for the three translational reading frames preceding the FLAG coding sequence are provided.pFLAG-CMV-5a expression vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of C-terminal FLAG fusion proteins in mammalian cells.The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-fusion constructs.The multiple cloning region of the pFLAG-CMV-5a expression vector is not preceded by a translational initiation signal. A translational start sequence3 must be included with the insert DNA.pFLAG-CMV-5a expression vector is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells.p-FLAG-CMV-5a, p-FLAG-CMV-5b, and p-FLAG-CMV-5c Expression Vectors used for the expression of C-terminal FLAG® tagged proteins in mammalian cells. pFLAG-CMV-5 a,b,c Expression Vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of C-terminal FLAG-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen. The a, b, and c vector series makes all three reading frames available for every restriction site in the MCS.
Product DescriptionVersions of the pFLAG-CMV-5 expression vector for the three translational reading frames preceding the FLAG coding sequence are provided.pFLAG-CMV-5a expression vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of C-terminal FLAG fusion proteins in mammalian cells.The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-fusion constructs.The multiple cloning region of the pFLAG-CMV-5a expression vector is not preceded by a translational initiation signal. A translational start sequence3 must be included with the insert DNA.pFLAG-CMV-5a expression vector is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells.p-FLAG-CMV-5a, p-FLAG-CMV-5b, and p-FLAG-CMV-5c Expression Vectors used for the expression of C-terminal FLAG® tagged proteins in mammalian cells. pFLAG-CMV-5 a,b,c Expression Vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of C-terminal FLAG-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen. The a, b, and c vector series makes all three reading frames available for every restriction site in the MCS.
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联系方式：
免费订购电话:400-800-2947.
010-53513060
电邮:Biovector@163.com
NTCCbio@163.com
订购QQ：1843439339
BioVector NTCC典型培养物保藏中心主页http://www.biovector.net
资源查询网址http://Biovector.1688.com
博客http://biovector.blog.163.com
版权所有：BioVector NTCC保藏中心
禁止未经允许的拷贝和修改，侵权必究！