载体基本信息

出品公司: SIGMA

载体名称: pFLAG-CMV-2

质粒类型: 哺乳动物表达载体

高拷贝/低拷贝: 低拷贝

克隆方法: 多克隆位点；限制性内切酶

启动子: CMV

载体大小: 4679 bp

5 测序引物及序列: CMV Forward：CGCAAATGGGCGGTAGGCGTG

3 测序引物及序列: hGH-pA-R：CCAGCTTGGTTCCCAATAGA

载体标签: FLAG tag (N-末端）

载体抗性: 氨苄青霉素

筛选标记: 无

克隆菌株: --

宿主细胞（系）: 常用细胞系，如293、Hela等

备注: CMV启动子驱动目的蛋白高水平表达；
FLAG标签含8个氨基酸残基（DYKDDDDK），
一般位于融合蛋白的外表面，具有高度免疫灵敏性，便于检测和纯化目的蛋白；

产品目录号: E7398

稳定性: 瞬表达

组成型/诱导型: 组成型

病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

载体简介

载体序列

LOCUS       pFLAG-CMV-2	4679 bp 	DNA	SYNDEFINITION  pFLAG-CMV-2ACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..4679                     /organism="pFLAG-CMV-2"                     /mol_type="other DNA"     misc_feature    complement(12..155)                     /label="lacZ_a"     misc_feature    126..148                     /label="M13_pUC_fwd_primer"     promoter        141..157                     /label="M13_forward20_primer"     promoter        319..895                     /label="CMV_immearly_promoter"     misc_feature    398..685                     /label="CAG_enhancer"     promoter        841..912                     /label="CMV_promoter"     misc_feature    852..872                     /label="CMV_fwd_primer"     misc_feature    896..915                     /label="pCEP_fwd_primer"     terminator      1030..1496                     /label="hGH_PA_terminator"     misc_feature    complement(1666..1686)                     /label="pBABE_3_primer"     misc_feature    complement(1672..1887)                     /label="SV40_enhancer"     promoter        1684..1952                     /label="SV40_promoter"     rep_origin      1851..1928                     /label="SV40_origin"     misc_feature    1913..1932                     /label="SV40pro_F_primer"     promoter        complement(2039..2057)                     /label="T7_promoter"     misc_feature    complement(2084..2106)                     /label="M13_pUC_rev_primer"     promoter        complement(2120..2149)                     /label="lac_promoter"     gene            complement(3232..4092)                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             complement(3232..4092)                     /label="ORF frame 3"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        complement(4134..4162)                     /label="AmpR_promoter"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     rep_origin      4241..4547                     /label="f1_origin"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"ORIGIN    1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct   61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg  121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa  181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta  241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc  301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg  361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc  421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat  481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc  541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga  601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg  661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac  721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt  781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc  841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc  901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct  961 tgcggccgcg aattcatcga tagatctgat atcggtacca gtcgactcta gaggatcccg 1021 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag 1081 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct 1141 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa 1201 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc 1261 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt 1321 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg 1381 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt 1441 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga 1501 ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg 1561 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca 1621 ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt 1681 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 1741 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 1801 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 1861 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 1921 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 1981 gaggcctagg cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaattccc 2041 tatagtgagt cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 2101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 2161 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 2221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 2281 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 2341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 2401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 2461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 2521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 2581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 2641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 2701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 2761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 2821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 2881 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 2941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 3001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 3061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 3121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 3181 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 3241 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 3301 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 3361 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 3421 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 3481 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 3541 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 3601 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 3661 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 3721 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 3781 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 3841 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 3901 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 3961 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 4021 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 4081 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 4141 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 4201 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 4261 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 4321 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 4381 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 4441 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 4501 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 4561 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 4621 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattt//

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