Oct4 (Octamer-binding protein 4) is a homeodomain transcription factor of the POU family, expressed in embryonic stem (ES) cells and embryonic carcinoma (EC) cells.Oct4 is critically involved in the signaling pathway for maintaining self-renewal and pluripotency of ES cells. Oct4 has two distinct DNA binding domains which independently bind half-sites of the canonical octamer motif. This flexibility enables Oct4 to bind with distinct DNA motifs by forming heterodimers with other transcription factors or by forming homodimers in several conformations. Human Oct4 contains a 75aa POU specific (POUS) domain and a 60aa POU-Homeo-( POUH) domain connected by a linker region. Human Oct4 specifically interact with Octamer motif ATGCAAAT.Moreover,two proline-rich domains in N-terminal and C-terminal regions are critical for Oct4 transactivation. Oct4 regulates a number of target genes and it has been shown to work in concert with other transcription factors including Sox2 as well as Nanog to support stem cell potency and self-renewal.

·Description
Recombinant Human Oct4 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 360 amino acids and having a molecular mass of 38.6kDa. The Recombinant Human Oct4 is produced as N-terminal TAT(transcriptional activator protein) fusion protien.

·Quality Control

Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Gly-His-Leu.

Endotoxin:Less than 0.1ng/μg (1.0EU/μg) of rHuOct4. as determined by LAL test.

 

·Formulation
SteriLyophilized from a 0.2um filtered protein solution in PBS.

·Reconstitution
It is recommended to reconstitute the lyophilized rHuOct4 in sterile PBS not less than 100μg/ml, which can then be further diluted to other solutions.

·Storage
Lyophilized rHuOct4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human Oct4 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples with 0.1% HSA or BSA are stable up to 3 months below -18°C for future use.