
delta PE OCT4-GFP-2A-PURO transgenic reporter
(Plasmid #52380)
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PURPOSE
BAC recombineering generated construct, used as a reporter for OCT4 expression
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BACKBONE
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Vector backbone
minimal Amp vector - Backbone size w/o insert (bp)2278
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Vector type
Mammalian Expression -
Selectable markers
Puromycin
GROWTH IN BACTERIA
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Bacterial Resistance(s)
Ampicillin -
Growth Temperature
37°C -
Growth Strain(s)
DH5alpha -
Copy number
High Copy
SEQUENCE INFORMATION
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Depositor Sequences
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Addgene Sequences
GENE/INSERT 1
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Gene/Insert name
GFP-2A-puro -
Species
Synthetic
CLONING INFORMATION FOR GENE/INSERT 1
GENE/INSERT 2
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Gene/Insert name
OCT4 -
Species
H. sapiens (human) -
Insert Size (bp)
17400 -
Mutation
deleted -1620 - -1011 upstream from OCT4 ATG -
Entrez Gene
POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
CLONING INFORMATION FOR GENE/INSERT 2
- Cloning methodLigation Independent Cloning
- 5′ sequencing primerTTGTCTGTAAGCGGATGCCGG
- 3′ sequencing primerCGCCTTTGAGTGAGCTGATACCG
(Common Sequencing Primers)
How to cite this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
delta PE OCT4-GFP-2A-PURO transgenic reporter was a gift from Jacob Hanna (Addgene plasmid # 52380)
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For your References section:
Derivation of novel human ground state naive pluripotent stem cells. Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH. Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30. 10.1038/nature12745 PubMed 24172903
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免费订购电话:400-800-2947.
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电邮:Biovector@163.com
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订购QQ:1843439339
BioVector NTCC典型培养物保藏中心主页http://www.biovector.net
资源查询网址http://Biovector.1688.com
博客http://biovector.blog.163.com
点击下载 产品分类目录简介
版权所有:BioVector NTCC保藏中心
禁止未经允许的拷贝和修改,侵权必究!