目录号	规格	价格	说明书下载
C417A	2μg	400元
C417B	10μg	1800元
C417C	50μg	4500元

 

Macrophage colony-stimulating factor 1, also known as M-CSF,CSF-1, Lanimostim, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. M-CSF is a cytokine and acts as a primary regulator of macrophage survival, proliferation and differentiation. It is also essential for the survival and proliferation of osteoclast progenitors. M-CSF plays important roles in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Recombinant M-CSF containing N terminal portion of M-CSF is sufficient for interaction with the M-CSF receptor. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species specific.

·Description
Recombinant Human M-CSF produced by transiently transfected human cell consists of sequence (Accession#P09603)(Glu33-Arg255) of human M-CSF fused with a polyhistidine tag at the C-terminus.

·Quality Control

Biological activity:The ED50 of rHuM-CSF is 0.5-1.5 ng/mL,as measured in a cell proliferation assay using M-NFS-60 mouse myeloid cells.

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: Predicted N terminal amino acid is Glu33.

Endotoxin:Less than 0.1ng/μg (1EU/μg) of rHuM-CSF.

 

·Formulation
 Sterile and Lyophilized from a 0.2 μm filtered solution in PBS.

·Reconstitution
It is recommended to reconstitute the lyophilized rHuM-CSF in sterile water not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

·Storage
Lyophilized rHuM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuM-CSF should be stored at 4°C between 2-7 days and aliquots of reconstituted samples containing 0.1% BSA are stable up to 3 months below -18°C for future use.