Homo sapiens mucin 1, cell surface associated (MUC1)
英文名称： Homo sapiens mucin 1, cell surface associated (MUC1)
型号:null    产品货号： GE0152
价格:请致电：010-57128832,18610462672
品牌: 深圳
试剂级别： 分子生物学级

NM_002456 Homo sapiens mucin 1, cell surface associated (MUC1) 质粒载体pLVX-MCS

链非糖基化多肽链，分子量为
15.8kDa。

·质量控制
生物学活性：诱导U87MG人成胶质瘤细胞激活STAT3实验测定ED50为5ng/ml。
纯度： 由以下方法测定纯度大于95.0：
（a）高效液相色谱（RP-HPLC）；
（b）还原和非还原SDS-PAGE，银染。
分子量: 还原 SDS-PAGE，15.8KDa +/-10。
等电点： IEF检测，等电点为 4.5-5.5。
氨基酸序列：N末端氨基酸序列为：Ser-His-Thr-Leu-Pro。
内毒素残留：LAL检测，小于0.1ng/μg (1 EU/μg) 。

·制剂成份
本产品为无菌冻干粉剂。

·复溶
建议将冻干hIL-31溶解在无菌的PBS中，浓度不低于100μg/ml，以待进一步稀释至工作浓度。

·保存条件
请使用手动化霜冰箱，并避免反复冻融。
无菌冻干粉剂：可在室温下存放3周，对其活性无明显影响；长期保存请存放于-18℃以下，可稳定保存至少
一年。
复溶后的细胞因子（请按推荐的复溶条件进行，如需进一步稀释，请查考相关文献）：4℃可稳定储存 2-7
天；长期保存请分装后存放于-18℃以下，可稳定保存至少三个月。
*仅供科研使用，不能应用于人体。*

·其他说明
详细内容请参考说明书

The individual subunits have a relative molecular mass each of 17.4 Daltons. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.

·Quality Control
Biological activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 3.0 x 107 IU/mg.
Purity: Greater than 95% as determined by
(a) Analysis by SEC-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Molecular weight: 17.3 KD+/-10% determined by reduced SDS-PAGE.
Isoelectric Point: the main zone between 4.0~5.0 analysis by IEF.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-.
Endotoxin: Less than 0.1ng/μg (1 IEU/μg) as determined by LAL test.

·Formulation
Lyophilized from a 0.2 μm filtered solution in PBS.

·Reconstitution
It is recommended to reconstitute the lyophilized recombinant Rabbit TNF-alpha in PBS containing at least 0.1% human or bovine serum albumin. not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

·Storage
Lyophilized samples are stable for up to twelve months from date of receipt at -20°C to -70°C.

Please avoid freeze-thaw cycles.