| 出品公司: | SIGMA |
|---|---|
| 载体名称: | pFLAG-CMV-4 |
| 质粒类型: | 哺乳动物表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 多克隆位点；限制性内切酶 |
| 启动子: | CMV |
| 载体大小: | 6271 bp |
| 5 测序引物及序列: | CMV Forward：CGCAAATGGGCGGTAGGCGTG |
| 3 测序引物及序列: | CCAGCTTGGTTCCCAATAGA |
| 载体标签: | FLAG tag (N-末端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | -- |
| 宿主细胞（系）: | 常用细胞系，如293、Hela等 |
| 备注: | CMV启动子驱动目的蛋白高水平表达； FLAG标签含8个氨基酸残基（DYKDDDDK）， 一般位于融合蛋白的外表面，具有高度免疫灵敏性，便于检测和纯化目的蛋白； 用EK蛋白酶可将3XFLAG标签切除。 |
| 产品目录号: | E7158 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
英文名称： pFLAG-CMV-4载体哺乳细胞表达载体质粒图谱序列抗性说明书价格报价
试剂级别： 分子生物学级
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
pFLAG-CMV-4 is a 6.2 kb expression vector used for transient or stable expression in mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression of a properly inserted open reading frame as an N-terminal Met-FLAG fusion protein.The pFLAG-CMV-4 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E.coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available for Sigma Aldrich. pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.PrincipleThe promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-fusion constructs. The aminoglycoside phosphotransferase II gene (neo-r) confers resistance to aminoglycosides such as G 418 allowing for selection of stable transfectants.
pFLAG-CMV-4 is a 6.2 kb expression vector used for transient or stable expression in mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression of a properly inserted open reading frame as an N-terminal Met-FLAG fusion protein.The pFLAG-CMV-4 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E.coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available for Sigma Aldrich. pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.PrincipleThe promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-fusion constructs. The aminoglycoside phosphotransferase II gene (neo-r) confers resistance to aminoglycosides such as G 418 allowing for selection of stable transfectants.
载体序列
LOCUS pFLAG-CMV-4 6271 bp DNA SYNDEFINITION pFLAG-CMV-4ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..6271 /or
LOCUS pFLAG-CMV-4 6271 bp DNA SYNDEFINITION pFLAG-CMV-4ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..6271 /or
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