载体基本信息

出品公司:
Invitrogen

载体名称: pBudCE4.1

质粒类型: 哺乳动物细胞表达载体

高拷贝/低拷贝: --

启动子: CMV or EF-1a

克隆方法: 多克隆位点，限制性内切酶

载体大小: 4595 bp

5 测序引物及序列: CMV Forward: 5d[TAATACGACTCACTATAGGG]3
EF-1a Forward:5d[TCAAGCCTCAGACAGTGGTTC]3

3 测序引物及序列: --

载体标签: 6XHis； c-myc epitope

载体抗性: zeocin

筛选标记: --

备注: 克隆宿主菌E.coli TOP10 ；DH5alpha T1 。
该载体能够独立表达两种基因，是双表达载体。表达水平高。

产品目录号: V86420

稳定性: --

组成型: 组成型

病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

载体简介

pBudCE4.1 载体使用时的注意事项：       现在市面上出售的pBudCE4.1载体，在多克隆位点位置都有突变，我们保证我们载体质粒序列的正确性，请大家放心购买！       1.在使用E.coli 克隆菌株时最好使用不含Tn5基因的菌株，如TOP10和DH5α T1；       2.采用电转法时最好用TOP10；       3.Zeocin 工作浓度推荐25 mg/L ~ 50 mg/L

载体序列

LOCUS       pBudCE4.1	4595 bp 	DNA	SYNDEFINITION  pBudCE4.1ACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..4595                     /organism="pBudCE4.1"                     /mol_type="other DNA"     promoter        11..627                     /label="CMV_immearly_promoter"     misc_feature    90..377                     /label="CAG_enhancer"     misc_feature    544..564                     /label="CMV_fwd_primer"     promoter        638..656                     /label="T7_promoter"     misc_feature    complement(823..842)                     /label="EBV_rev_primer"     misc_feature    complement(1063..1437)                     /label="sh_ble"     gene            complement(1066..1437)                     /label="bleo"                     /gene="bleo"     promoter        complement(1438..1505)                     /label="EM7_promoter"     misc_feature    complement(1619..1638)                     /label="SV40pro_F_primer"     rep_origin      complement(1623..1700)                     /label="SV40_origin"     misc_feature    1670..1861                     /label="SV40_enhancer"     CDS             complement(1877..2791)                     /label="ORF frame 2"                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS                     IADPSPQLLGDCGRCALCPLTGTGASRS*"     promoter        1884..3051                     /label="EF1a_promoter"                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS                     IADPSPQLLGDCGRCALCPLTGTGASRS*"     misc_feature    2999..3019                     /label="EF1a_fwd_primer"                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS                     IADPSPQLLGDCGRCALCPLTGTGASRS*"     misc_feature    complement(3218..3235)                     /label="BGH_rev_primer"                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS                     IADPSPQLLGDCGRCALCPLTGTGASRS*"     terminator      3221..3448                     /label="bGH_PA_terminator"                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS                     IADPSPQLLGDCGRCALCPLTGTGASRS*"     rep_origin      complement(3565..4184)                     /label="pBR322_origin"                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS                     IADPSPQLLGDCGRCALCPLTGTGASRS*"     misc_feature    complement(4315..4337)                     /label="pGEX_3_primer"                     /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL                     TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP                     RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR                     GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG                     TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS                     IADPSPQLLGDCGRCALCPLTGTGASRS*"ORIGIN    1 GCGCGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG   61 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT  121 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC  181 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGACTATTT ACGGTAAACT GCCCACTTGG  241 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT  301 GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA  361 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC  421 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA  481 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT  541 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC  601 TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA  661 CCCAAGCTTG CATTCCTGCA GGTCGACATC GATCTTAAGC AGTACTTCTA GAGGATCCGA  721 ACAAAAACTC ATCTCAGAAG AGGATCTGAA TATGCATACC GGTCATCATC ACCATCACCA  781 TTGAGTTTGA TCCCCGGGAA TTCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC  841 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA  901 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTGGGGTGG GCGAAGAACT CCAGCATGAG  961 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 1021 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTAGCACG TGTCAGTCCT GCTCCTCGGC 1081 CACGAAGTGC ACGCAGTTGC CGGCCGGGTC GCGCAGGGCG AACTCCCGCC CCCACGGCTG 1141 CTCGCCGATC TCGGTCATGG CCGGCCCGGA GGCGTCCCGG AAGTTCGTGG ACACGACCTC 1201 CGACCACTCG GCGTACAGCT CGTCCAGGCC GCGCACCCAC ACCCAGGCCA GGGTGTTGTC 1261 CGGCACCACC TGGTCCTGGA CCGCGCTGAT GAACAGGGTC ACGTCGTCCC GGACCACACC 1321 GGCGAAGTCG TCCTCCACGA AGTCCCGGGA GAACCCGAGC CGGTCGGTCC AGAACTCGAC 1381 CGCTCCGGCG ACGTCGCGCG CGGTGAGCAC CGGAACGGCA CTGGTCAACT TGGCCATGGT 1441 TTAGTTCCTC ACCTTGTCGT ATTATACTAT GCCGATATAC TATGCCGATG ATTAATTGTC 1501 AACACGTGCT GATCAGATCC GAAAATGGAT ATACAAGCTC CCGGGAGCTT TTTGCAAAAG 1561 CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC AGAGGCGGCC 1621 TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG 1681 GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG 1741 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT 1801 GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT 1861 CCACACCCTC GTCGAGCTAG CTTCGTGAGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC 1921 ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG 1981 AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA 2041 GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG 2101 GTTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC 2161 GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTCCAGTACG TGATTCTTGA 2221 TCCCGAGCTG GAGCCAGGGG CGGGCCTTGC GCTTTAGGAG CCCCTTCGCC TCGTGCTTGA 2281 GTTGAGGCCT GGCCTGGGCG CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC 2341 TGTCTCGCTG CTTTCGATAA GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG 2401 CTTTTTTTCT GGCAAGATAG TCTTGTAAAT GCGGGCCAGG ATCTGCACAC TGGTATTTCG 2461 GTTTTTGGGC CCGCGGCCGG CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG 2521 CGGGGCCTGC GAGCGCGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT 2581 GCTCTGGTGC CTGGCCTCGC GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC 2641 CGGTCGGCAC CAGTTGCGTG AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TCCAGGGGGC 2701 TCAAAATGGA GGACGCGGCG CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA 2761 AGGGCCTTTC CGTCCTCAGC CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC 2821 AGGCACCTCG ATTAGTTCTG GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG 2881 TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG 2941 CACTTGATGT AATTCTCGTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC 3001 AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AACACGTGGT 3061 CGCGGCCGCT TCGAAGGTAC CAGCACAGTG GACTCGAGAG ATCTGGCCGG CTGGGCCCGT 3121 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT 3181 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC 3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG 3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG TGGCGGTAAT 3481 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 3541 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 3601 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 3661 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 3721 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 3781 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 3841 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 3901 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 3961 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 4021 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 4081 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 4141 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 4201 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGACATTAA CCTATAAAAA 4261 TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG 4321 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA 4381 AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC 4441 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATATGCGGT GTGAAATACC GCACAGATGC 4501 GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA 4561 GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCA

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