LOCUS pRSET/CFP 3600 bp DNA SYNDEFINITION pRSET/CFPACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..3600 /organism="pRSET/CFP" /mol_type="other DNA" promoter 9..27 /label="T7_promoter" misc_feature 64..80 /label="T7_transl_en_RBS" CDS 89..928 /label="ORF frame 2" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 101..118 /label="6xHIS" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 121..139 /label="Xpress_fwd_primer" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 122..154 /label="T7_leader" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 122..154 /label="T7 Tag" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 158..181 /label="Xpress_EK" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAHFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" CDS 187..942 /label="ORF frame 1" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" gene 212..925 /label="ECFP" /gene="ECFP" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature complement(254..275) /label="EGFP_N_primer" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature 392..421 /label="Y66W (ECFP)" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature 862..883 /label="EGFP_C_primer" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" terminator 948..1076 /label="T7_terminator" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature complement(990..1008) /label="T7_Terminal_primer" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" rep_origin 1165..1471 /label="f1_origin" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" promoter 1664..1692 /label="AmpR_promoter" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" gene 1734..2594 /label="Ampicillin" /gene="Ampicillin" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPDLGRAVLQPLPRPHEAARLLQVRHARRLRP GAYHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLHQPQRLY HRRQAEERHQGPLQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" CDS 1734..2594 /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 2749..3368 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 CGCGAAATTA ATACGACTCA CTATAGGGAG ACCACAACGG TTTCCCTCTA GAAATAATTT 61 TGTTTAACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT CATCATCATC ATCATCATGG 121 TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG ATGACGATAA 181 GGATCGATGG GGATCCGAAT TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG 241 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAGGT TCAGCGTGTC 301 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC 361 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTGGG GCGTGCAGTG 421 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA 481 AGGCTACGTC CAGGAGCGTA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC 541 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT 601 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACATCA GCCACAACGT 661 CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC CACTTCAAGA TCCGCCACAA 721 CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA 781 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA 841 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC 901 TCTCGGCATG GACGAGCTGT ACAAGTAACT CGAGAAGCTT GATCCGGCTG CTAACAAAGC 961 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 1021 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATCTGG 1081 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 1141 GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 1201 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 1261 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 1321 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 1381 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 1441 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 1501 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 1561 AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAGGTGGC ACTTTTCGGG 1621 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 1681 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 1741 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 1801 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 1861 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 1921 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 1981 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 2041 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 2101 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 2161 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 2221 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 2281 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 2341 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 2401 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 2461 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 2521 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 2581 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 2641 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 2701 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 2761 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 2821 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 2881 GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC 2941 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 3001 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 3061 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 3121 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG 3181 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 3241 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 3301 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 3361 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 3421 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 3481 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC 3541 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGG ATCTCGATCC//
