| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pRSET-BFP |
| 质粒类型: | 大肠杆菌表达载体；克隆载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 启动子: | T7 |
| 载体大小: | 3.6 kb |
| 5 测序引物及序列: | T7 Forward |
| 3 测序引物及序列: | -- |
| 载体标签: | His Tag (6x)；Xpress Epitope Tag |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 克隆菌株: | DH5alpha，TOP10F |
| 表达菌株: | BL21(DE3)系列，推荐BL21Star（DE3) |
| 备注: | pRSET-BFP载体的功能是克隆荧光报告基因BFP或荧光蛋白BFP的表达和纯化，菌体裂解物在紫外灯下肉眼可见荧光。 含 EK (Enterokinase)识别位点 |
| 产品目录号: | V35420 |
| 稳定性: | -- |
| 组成型/诱导型: | 诱导型（IPTG) |
| 病毒/非病毒: | 非病毒 |
英文名称： pRSET-BFP大肠杆菌载体
试剂级别： 分子生物学级
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
The pRSET-EmGFP, pRSET-CFP and pRSET-BFP vectors allow you to fuse a protein of interest to the widely used and well-characterized fluorescent proteins from the jellyfish Aequorea victoria (1,2) using the pRSET expression vector. These vectors contain the next-generation EGFP, Emerald Green Fluorescent Protein (EmGFP), which has been further optimized for mammalian expression, as well as Cyan (CFP) and Blue (BFP) Fluorescent Proteins for simple, noninvasive detection of recombinant protein.
The pRSET-EmGFP, pRSET-CFP and pRSET-BFP vectors allow you to fuse a protein of interest to the widely used and well-characterized fluorescent proteins from the jellyfish Aequorea victoria (1,2) using the pRSET expression vector. These vectors contain the next-generation EGFP, Emerald Green Fluorescent Protein (EmGFP), which has been further optimized for mammalian expression, as well as Cyan (CFP) and Blue (BFP) Fluorescent Proteins for simple, noninvasive detection of recombinant protein.
载体序列
LOCUS pRSET/BFP 3600 bp DNA SYNDEFINITION pRSET/BFPACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..3600 /organism="pRSET/BFP" /mol_type="other DNA" promoter 9..27 /label="T7_promoter" misc_feature 64..80 /label="T7_transl_en_RBS" CDS 89..928 /label="ORF frame 2" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 101..118 /label="6xHIS" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 121..139 /label="Xpress_fwd_primer" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 122..154 /label="T7_leader" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 122..154 /label="T7 Tag" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 158..181 /label="Xpress_EK" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" CDS 187..942 /label="ORF frame 1" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" gene 212..925 /label="EGFP" /gene="EGFP" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature complement(254..275) /label="EGFP_N_primer" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature 862..883 /label="EGFP_C_primer" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" terminator 948..1076 /label="T7_terminator" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature complement(990..1008) /label="T7_Terminal_primer" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" rep_origin 1165..1471 /label="f1_origin" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" promoter 1664..1692 /label="AmpR_promoter" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" gene 1734..2594 /label="Ampicillin" /gene="Ampicillin" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" CDS 1734..2594 /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 2749..3368 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 CGCGAAATTA ATACGACTCA CTATAGGGAG ACCACAACGG TTTCCCTCTA GAAATAATTT 61 TGTTTAACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT CATCATCATC ATCATCATGG 121 TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG ATGACGATAA 181 GGATCGATGG GGATCCGAAT TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG 241 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC 301 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC 361 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGAGCCACG GCGTCCAGTG 421 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA 481 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC 541 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT 601 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT 661 CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGCG AACTTCAAGA TCCGCCACAA 721 CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA 781 CGGCCCCGTG CTGCTGCCCG ACAGCCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA 841 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC 901 TCTCGGCATG GACGAGCTGT ACAAGTAACT CGAGAAGCTT GATCCGGCTG CTAACAAAGC 961 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 1021 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATCTGG 1081 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 1141 GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 1201 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 1261 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 1321 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 1381 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 1441 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 1501 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 1561 AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAGGTGGC ACTTTTCGGG 1621 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 1681 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 1741 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 1801 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 1861 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 1921 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 1981 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 2041 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 2101 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 2161 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 2221 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 2281 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 2341 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 2401 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 2461 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 2521 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 2581 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 2641 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 2701 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 2761 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 2821 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 2881 GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC 2941 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 3001 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 3061 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 3121 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG 3181 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 3241 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 3301 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 3361 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 3421 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 3481 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC 3541 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGG ATCTCGATCC//
LOCUS pRSET/BFP 3600 bp DNA SYNDEFINITION pRSET/BFPACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..3600 /organism="pRSET/BFP" /mol_type="other DNA" promoter 9..27 /label="T7_promoter" misc_feature 64..80 /label="T7_transl_en_RBS" CDS 89..928 /label="ORF frame 2" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 101..118 /label="6xHIS" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 121..139 /label="Xpress_fwd_primer" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 122..154 /label="T7_leader" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 122..154 /label="T7 Tag" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" misc_feature 158..181 /label="Xpress_EK" /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSEFATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV TTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL VNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE LYK*" CDS 187..942 /label="ORF frame 1" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" gene 212..925 /label="EGFP" /gene="EGFP" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature complement(254..275) /label="EGFP_N_primer" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature 862..883 /label="EGFP_C_primer" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" terminator 948..1076 /label="T7_terminator" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" misc_feature complement(990..1008) /label="T7_Terminal_primer" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" rep_origin 1165..1471 /label="f1_origin" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" promoter 1664..1692 /label="AmpR_promoter" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" gene 1734..2594 /label="Ampicillin" /gene="Ampicillin" /translation="MGIRIRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGR CHLRQADPEVHLHHRQAARALAHPRDHPEPRRPVLQPLPRPHEAARLLQVRHARRLRP GAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQR PQREARSHGPAGVRDRRRDHSRHGRAVQVTREA*" CDS 1734..2594 /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 2749..3368 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 CGCGAAATTA ATACGACTCA CTATAGGGAG ACCACAACGG TTTCCCTCTA GAAATAATTT 61 TGTTTAACTT TAAGAAGGAG ATATACATAT GCGGGGTTCT CATCATCATC ATCATCATGG 121 TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG ATGACGATAA 181 GGATCGATGG GGATCCGAAT TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG 241 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC 301 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC 361 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGAGCCACG GCGTCCAGTG 421 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA 481 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC 541 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT 601 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT 661 CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGCG AACTTCAAGA TCCGCCACAA 721 CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA 781 CGGCCCCGTG CTGCTGCCCG ACAGCCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA 841 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC 901 TCTCGGCATG GACGAGCTGT ACAAGTAACT CGAGAAGCTT GATCCGGCTG CTAACAAAGC 961 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 1021 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATCTGG 1081 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 1141 GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 1201 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 1261 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 1321 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 1381 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 1441 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 1501 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 1561 AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAGGTGGC ACTTTTCGGG 1621 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 1681 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 1741 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 1801 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 1861 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 1921 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 1981 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 2041 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 2101 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 2161 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 2221 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 2281 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 2341 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 2401 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 2461 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 2521 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 2581 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 2641 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 2701 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 2761 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 2821 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 2881 GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC 2941 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 3001 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 3061 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 3121 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG 3181 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 3241 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 3301 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 3361 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 3421 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 3481 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC 3541 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGG ATCTCGATCC//
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BioVector NTCC典型培养物保藏中心主页http://www.biovector.net
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BioVector NTCC典型培养物保藏中心拥有数十万种基因cDNA克隆库，包括人源、鼠源、兔源、模式动植物源、微生物源，及稀缺基因资源。覆盖30,000个人全长基因的TrueClone cDNA克隆和超过25,000个TrueORF cDNA克隆。利用TrueORF cDNA克隆，我们开发了大量的全长人重组蛋白产品（哺乳动物细胞表达），可以进行蛋白功能的研究。另外，Biovector NTCC Inc.提供独特的基因表达产品，如TissueScan cancer qPCR array用于生物标记物的发现及鉴定。
33,000 种人TrueClones 全长人cDNA克隆（无标签）
5,000种小鼠 TrueClones 全长小鼠cDNA克隆（无标签）
25,000 种人 TrueORF 人ORF cDNA克隆（带标签）
12,000种小鼠 TrueORF 小鼠ORF cDNA克隆（带标签）
5,000种人重组蛋白全长的人重组蛋白（HEK293T细胞表达）
5,000 种一抗经鉴定的特异性识别人源蛋白的一抗产品
25,000 种人 HuSH-29 shRNA 预设计的shRNA 表达载体（人基因），用于基因沉默
20,000 种小鼠 HuSH-29 shRNA 预设计的shRNA 表达载体（小鼠基因），用于基因沉默
10,000种 VERIFY TaggedAntigen 过表达细胞裂解物（可作为抗原和检测分析标准品）
240 Luminex Multiplex Assays SNP、转录因子和microRNA分析
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