硫酸酯酶2(SULF2)重组蛋白

YB107Mu021
Sulfatase Sulfatase Sulfatase Sulfatase 2 (SULF2) (SULF2) (SULF2) (SULF2)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
硫酸酯酶2(SULF2)重组蛋白[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Phe337~Arg649 (Accession # Q8IWU5),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Endoplasmic reticulum. Golgi
apparatus, Golgi stack. Cell surface.
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
硫酸酯酶2(SULF2)重组蛋白Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 8.7
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 62.7kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
硫酸酯酶2(SULF2)重组蛋白that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
GS T S G S G H H H HH HSA G LVP R GS TA IG MK E T AA AK F E R Q HM DSP D L G T L EV
LFQGPLGSEF-FDIR VPFYVRGPNV EAGCLNPHIVLNIDLAPTIL DIAGLDIPAD MDGKSILKLL
DTERPVNRFH LKKKMRVWRD SFLVERGKLLHKRDNDKVDA QEENFLPKYQ RVKDLCQRAE
YQ TA CE Q L GQ KWQ CVE DAT G KL K L HK CK GPM R LGGS RA LS NLVPKYY GQG
SEACTCDSGD YKLSLAGRRK KLFKKKYKAS YVRSRSIRSVAIEVDGRVYH VGLGDAAQPR
NLTKRHWPGA PEDQDDKDGG DFSGTGGLPD YSAANPIKVTHRCYILENDT VQCDLDLYKS
LQAWKDHKLH IDHEIETLQN KIKNLREVR