***脂酰肌醇特异性***酯酶Cβ1(PLCβ1)重组蛋白

YB596Hu015
Recombinant Phospholipase C Beta 1, Phosphoinositide Specific (PLCb1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
***脂酰肌醇特异性***酯酶Cβ1(PLCβ1)重组蛋白PROPERTIES ]
Residues: Glu316~Gly476
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q9NQ66
Host: E. coli
Subcellular Location: Nucleus membrane,
Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
***脂酰肌醇特异性***酯酶Cβ1(PLCβ1)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.6
Predicted Molecular Mass: 21.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
***脂酰肌醇特异性***酯酶Cβ1(PLCβ1)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
EDMSQ PLSHYFINSS HNTYLTAGQL AGNSSVEMYR QVLLSGCRCV ELDCWKGRTA
EEEPVITHGF TMTTEISFKE VIEAIAECAF KTSPFPILLS FENHVDSPKQ QAKMAEYCRL
IFGDALLMEP LEKYPLESGV PLPSPMDLMY KILVKNKKKS HKSSEG